Gonzalez-Bulnes A, Garcia-Garcia R M, Souza C J H, Santiago-Moreno J, Lopez-Sebastian A, Cocero M J, Baird D T
Departamento de Reproduccion Animal, INIA, Madrid, Spain.
Reprod Domest Anim. 2002 Dec;37(6):357-61. doi: 10.1046/j.1439-0531.2002.00385.x.
Objectives of this study were to characterize patterns of follicular development in sheep superovulated with purified follicle stimulating hormone (FSH) (OVAGEN, ICP, Auckland, New Zealand) and to determine its influence on preovulatory events (onset of the oestrus behaviour and timing of the preovulatory luteinizing hormone surge) and ovarian response (ovulation rate and embryo yield). Number and size of all >/= 23 mm follicles from the first FSH injection to withdrawal of progestagen sponges was determined by transrectal ultrasonography just prior to every FSH injection in nine Manchega ewes superovulated with eight decreasing doses (ml) (1.5 x 3, 1.25 x 2 and 1 x 3) of OVAGEN injected twice daily from 60 h before to 24 h after the withdrawal of 40 mg fluorogestone acetate sponges. Oestrous detection and jugular blood sampling for LH radioimmunoassay were performed every 3 h from 14 to 53 h after sponge removal and ovulation rate and number of embryos were determined 4 days after progestagen withdrawal. Administration of OVAGEN induced a significant rise (p < 0.0005) in the number of follicles >/= 4 mm in size because of an increased growth in size of follicles from the first FSH injection to sponge removal, an increase in the number of newly detected follicles from 12 to 36 h of the first FSH dose (p < 0.005) and a decrease in regression rate from 24 h (p < 0.001). The number of follicles 2-3 mm in size at first FSH dose (10.4 +/- 1.5) was positively correlated with the number of >/= 4 mm follicles at 0 h (19.0 +/- 2.7, p < 0.01). A higher number of >/= 4 mm follicles at 0 h was related with an earlier appearance of oestrus (31.5 +/- 1.5 h, p = 0.08) and LH surge (45.0 +/- 2.3 h, p < 0.005), and a higher ovulation rate (18.2 +/- 3.8, p < 0.005). On the other hand, the rate of embryo recovery was decreased in ewes with earlier preovulatory LH peaks (p < 0.005), with a shorter interval between oestrus and LH peak (p < 0.05).
本研究的目的是描述用纯化的促卵泡激素（FSH）（OVAGEN，ICP，奥克兰，新西兰）进行超排的绵羊卵泡发育模式，并确定其对排卵前事件（发情行为的开始和排卵前促黄体生成素高峰的时间）以及卵巢反应（排卵率和胚胎产量）的影响。对9只曼彻格母羊用8个递减剂量（毫升）（1.5×3、1.25×2和1×3）的OVAGEN进行超排，从取出40毫克醋酸氟孕酮海绵前60小时至取出后24小时，每天两次注射，在每次FSH注射前，通过经直肠超声检查确定从首次FSH注射到取出孕激素海绵期间所有≥23毫米卵泡的数量和大小。从取出海绵后14至53小时，每3小时进行一次发情检测和颈静脉采血用于LH放射免疫测定，并在取出孕激素后4天确定排卵率和胚胎数量。由于从首次FSH注射到取出海绵期间卵泡大小增加、在首次FSH剂量的12至36小时新检测到的卵泡数量增加（p<0.005）以及从24小时起退化率降低（p<0.001），OVAGEN的给药导致≥4毫米大小的卵泡数量显著增加（p<0.0005）。首次FSH剂量时2 - 3毫米大小的卵泡数量（10.4±1.5）与0小时时≥4毫米卵泡的数量（19.0±2.7，p<0.01）呈正相关。0小时时较高数量的≥4毫米卵泡与较早出现发情（31.5±1.5小时，p = 0.08）和LH高峰（45.0±2.3小时，p<0.005）以及较高的排卵率（18.2±3.8，p<0.005）有关。另一方面，排卵前LH峰值较早的母羊胚胎回收率降低（p<0.005），发情和LH峰值之间的间隔较短（p<0.05）。
