Ma Hsin-Chieh, Ke Cheng-Hung, Hsieh Tsai-Yuan, Lo Shih-Yen
Institute of Medical Research1 and Department of Medical Technology2, Tzu Chi University, 701, Section 3, Chung-Yang Road, Hualien, Taiwan 970, Republic of China.
Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China4.
J Gen Virol. 2002 Dec;83(Pt 12):3085-3092. doi: 10.1099/0022-1317-83-12-3085.
The interaction between the hepatitis C virus capsid protein and the envelope protein E1 has been demonstrated previously in vivo. To determine the binding region of the E1 protein with the capsid protein, this interaction was characterized in vitro. This study shows that the interaction between these proteins should occur in the endoplasmic reticulum membrane rather than in the cytosol and that the first hydrophobic domain of the E1 protein (aa 261-291) is important for the interaction with the capsid protein.
先前已在体内证实丙型肝炎病毒衣壳蛋白与包膜蛋白E1之间存在相互作用。为了确定E1蛋白与衣壳蛋白的结合区域，在体外对这种相互作用进行了表征。这项研究表明，这些蛋白之间的相互作用应该发生在内质网膜而非细胞质中，并且E1蛋白的第一个疏水结构域（氨基酸261 - 291）对于与衣壳蛋白的相互作用很重要。
