多胺辅助从带有顺式二醇的通用载体上快速且干净地切割寡核苷酸。
Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support.
作者信息
Kumar P, Dhawan G, Chandra R, Gupta K C
机构信息
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology (formerly Centre for Biochemical Technology), Mall Road, Delhi University Campus, Delhi 110 007, India.
出版信息
Nucleic Acids Res. 2002 Dec 1;30(23):e130. doi: 10.1093/nar/gnf130.
Abstract
Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.
摘要
已开发出多胺辅助的脱保护条件，用于从带有顺式二醇基团的通用聚合物载体上快速、干净地切割寡核苷酸链，使其能够与通过所有类型的亚磷酰胺合成子进行的现代寡核苷酸合成兼容，包括带有对碱不稳定保护基团的合成子。结果发现，合成的寡核苷酸在高效液相色谱（HPLC）上的保留时间、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）上的质量以及聚合酶链反应（PCR）扩增中的生物活性方面，与相应的标准寡聚物相当。
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