[Establishment of a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and its biological characteristics].

BACKGROUND & OBJECTIVE: Drug resistance is a popular topic in tumor study. The drug-resistant cell line established in vitro is generally used as the research model. To explore the mechanism of resistance of hepatocellular carcinoma(HCC) to cisplatin, we designed this study to establish a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and study its characteristics.

METHODS

A resistant HCC cell line (QGY/cDDP) was established gradually by increasing dose of cisplatin and intermittent administration; drug sensitivity was detected by MTT assay; the changes of its biological characteristics were determined using light microscopy, electron microscopy, cell counting, flow cytometry(FCM), and chromatosome analysis.

RESULTS

QGY/cDDP cell line was developed after 3 months with stable resistance to cisplatin and the resistance index was 10.81; QGY/cDDP cells exhibited cross-resistance to many other chemotherapeutic agents (5-Fuorouracil, epiadriamycin, etc). The morphology and chromatosome number of QGY/cDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while in G0/G1 phase increased compared with parent cells.

CONCLUSION

QGY/cDDP cell line shows the typical and stable resistant phenotype.