转基因动物中的抗体生产及其与其他系统的比较。
Antibody manufacture in transgenic animals and comparisons with other systems.
作者信息
Houdebine Louis Marie
机构信息
Biologie du Développement et Biotechnologies, Institut National de la Recherche Agronomique, 78352 Cedex, Jouy-en-Josas, France.
出版信息
Curr Opin Biotechnol. 2002 Dec;13(6):625-9. doi: 10.1016/s0958-1669(02)00362-2.
Abstract
Various forms of recombinant monoclonal antibodies are being used increasingly, mainly for therapeutic purposes. The isolation and engineering of the corresponding genes is becoming less of a bottleneck in the process; however, the production of recombinant antibodies is itself a limiting factor and a shortage is expected in the coming years. Milk from transgenic animals appears to be one of the most attractive sources of recombinant antibodies. None of the production systems presently implemented (CHO cells, insect cells infected by baculovirus, or transgenic animals and plants) has yet been optimized. This review describes the advantages of using milk for antibody production in comparison with the other systems.
摘要
各种形式的重组单克隆抗体正越来越多地被使用，主要用于治疗目的。相应基因的分离和工程化在这个过程中已不再是一个瓶颈；然而，重组抗体的生产本身就是一个限制因素，预计未来几年会出现短缺。转基因动物的乳汁似乎是重组抗体最有吸引力的来源之一。目前实施的任何生产系统（CHO细胞、被杆状病毒感染的昆虫细胞，或转基因动植物）都尚未得到优化。这篇综述描述了与其他系统相比，利用乳汁生产抗体的优势。
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