含α5亚基的γ-氨基丁酸A（GABA(A)）受体在海马体培养物中的GABA能突触处形成簇集。

alpha5 Subunit-containing GABA(A) receptors form clusters at GABAergic synapses in hippocampal cultures.

作者信息

Christie Sean B, de Blas Angel L

机构信息

Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06268, USA.

出版信息

Neuroreport. 2002 Dec 3;13(17):2355-8. doi: 10.1097/00001756-200212030-00037.

DOI:10.1097/00001756-200212030-00037

PMID:12488826

Abstract

We have used triple-label fluorescence immunocytochemistry to demonstrate that alpha5 subunit-containing GABA(A) receptors (GABA(A)Rs) form large clusters at GABAergic synapses in dendrites and axon initial segment of cultured hippocampal neurons. The large synaptic clusters of alpha5 subunit-containing GABA(A)Rs also contained alpha1, beta2/3, gamma2 GABA(A)R subunits and gephyrin. The alpha5 subunit-containing GABA(A)Rs also formed small clusters. The small clusters were not associated with GABAergic synapses and often did not co-localize with gephyrin.

摘要

我们运用三重标记荧光免疫细胞化学技术来证明，含α5亚基的γ-氨基丁酸A型受体（GABA(A)Rs）在培养的海马神经元树突和轴突起始段的GABA能突触处形成大簇。含α5亚基的GABA(A)Rs的大突触簇还包含α1、β2/3、γ2 GABA(A)R亚基和桥连蛋白。含α5亚基的GABA(A)Rs也形成小簇。这些小簇与GABA能突触无关，且通常不与桥连蛋白共定位。