I-A(g7)和I-A(d)四聚体的交叉反应性。

The cross-reactivity of I-A(g7) and I-A(d) tetramers.

作者信息

Peng Yong, Liu Chih-pin

机构信息

Department of Neurology, The Third Affiliated Hospital, Hunan Medical University, Changsha, Hunan 410013, China.

出版信息

Chin Med J (Engl). 2002 Oct;115(10):1579-81.

PMID:12490116

Abstract

OBJECTIVE

To investigate the cross-reactivity between glutamic acid decarboxylase (GAD)-I-A(g7) and I-A(d) tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-A(g7)) and diabetes-free Balb/c mice (I-A(d)).

METHODS

Two GAD peptide I-A(g7) and I-A(d) tetramers were generated and compared for phenotype and function of sorted GAD peptide I-A(g7) and I-A(d) tetramer-positive (tet+) T cells.

RESULTS

The cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-A(g7) and I-A(d) tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic and recombinant GAD peptides.

CONCLUSION

Although I-A(g7) and I-A(d) are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negatively charged residue at position beta57 that links to insulin-dependent diabetes mellitus.

摘要