甜菜黑色焦枯病毒（BBSV）的全核苷酸序列，坏死病毒属的一个新成员。
The complete nucleotide sequence of Beet black scorch virus (BBSV), a new member of the genus Necrovirus.
作者信息
Cao Y, Cai Z, Ding Q, Li D, Han C, Yu J, Liu Y
机构信息
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, China.
出版信息
Arch Virol. 2002 Dec;147(12):2431-5. doi: 10.1007/s00705-002-0896-1.
The complete nucleotide sequence of Beet black scorch virus (BBSV) was determined. The BBSV genome is composed of 3641 nucleotides and has similar organization with Tobacco necrosis virus D of 61% nucleotide identity. The 5'-proximal open reading frame (ORF) encodes a putative 23 kDa protein and a 82 kDa protein by reading-through of an amber termination codon. Three small ORFs located in the center of the genome may encode for a 4.2 kDa protein and two 7 kDa proteins. The 3'-proximal ORF encodes a 24.5 kDa protein equivalent in mass to the viral coat protein. Considering biological and molecular similarities with TNV, it is concluded that BBSV is a new member of the genus Necrovirus.
测定了甜菜黑焦病毒（BBSV）的完整核苷酸序列。BBSV基因组由3641个核苷酸组成，与烟草坏死病毒D具有相似的组织架构，核苷酸同一性为61%。5'近端开放阅读框（ORF）通过琥珀色终止密码子的通读编码一种推定的23 kDa蛋白和一种82 kDa蛋白。位于基因组中心的三个小ORF可能编码一种4.2 kDa蛋白和两种7 kDa蛋白。3'近端ORF编码一种24.5 kDa蛋白，其质量与病毒外壳蛋白相当。考虑到与烟草坏死病毒在生物学和分子方面的相似性，得出结论：BBSV是坏死病毒属的一个新成员。
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