通过反向PCR进行富含GC模板的扩增。DNA聚合酶和溶剂效应。 - Suppr

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超能文献

GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects.

作者信息

Moreau Alain, Wang Da Shen, Forget Steve, Duez Colette, Dusart Jean

机构信息

Sainte-Justine Hospital Research Center, Montreal, Canada.

出版信息

Methods Mol Biol. 2002;192:75-80. doi: 10.1385/1-59259-177-9:075.

DOI:10.1385/1-59259-177-9:075

PMID:12494639

Abstract

摘要

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GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects.

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引用本文的文献

Molecular cloning and expression of high GC-rich novel tumor suppressor gene HIC-1.

Mol Biotechnol. 2014 Nov;56(11):1040-8. doi: 10.1007/s12033-014-9783-8.

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相似文献

GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects.

Methods Mol Biol. 2002;192:75-80. doi: 10.1385/1-59259-177-9:075.

XL PCR amplification of long targets from genomic DNA.

Methods Mol Biol. 2002;192:37-51. doi: 10.1385/1-59259-177-9:037.

Long-range PCR.

Methods Mol Biol. 2002;187:51-5. doi: 10.1385/1-59259-273-2:051.

GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects.

Methods Mol Biol. 1997;67:47-53. doi: 10.1385/0-89603-483-6:47.

PCR amplification of GC-rich templates containing palindromic sequences using initial alkali denaturation.

Biotechniques. 1992 Apr;12(4):502-4.

Improved recombinant PCR mutagenesis procedure that uses alkaline-denatured plasmid template.

Biotechniques. 1995 Mar;18(3):376-8.

PCR. Principles, procedures, and parameters.

Methods Mol Biol. 2002;187:37-46. doi: 10.1385/1-59259-273-2:037.

PCR amplification of highly GC-rich DNA template after denaturation by NaOH.

Nucleic Acids Res. 1993 Nov 11;21(22):5283-4. doi: 10.1093/nar/21.22.5283.

[The polymerase chain reaction].

Ned Tijdschr Geneeskd. 1989 Sep 16;133(37):1825-9.

Recognition of the base pair-mimic nucleosides by DNA polymerases.

Nucleic Acids Symp Ser (Oxf). 2006(50):201-2. doi: 10.1093/nass/nrl100.

引用本文的文献

Molecular cloning and expression of high GC-rich novel tumor suppressor gene HIC-1.

Mol Biotechnol. 2014 Nov;56(11):1040-8. doi: 10.1007/s12033-014-9783-8.