Kote-Jarai Z, Durocher F, Edwards S M, Hamoudi R, Jackson R A, Ardern-Jones A, Murkin A, Dearnaley D P, Kirby R, Houlston R, Easton D F, Eeles R
Section of Cancer Genetics, The Institute of Cancer Research and Royal Marsden NHS Trust, Sutton, Surrey, UK.
Prostate Cancer Prostatic Dis. 2002;5(3):189-92. doi: 10.1038/sj.pcan.4500569.
Epidemiological studies have suggested an association between low selenium levels and the development of prostate cancer. Human cellular glutathione peroxidase I (hGPX1) is a selenium-dependent enzyme that protects against oxidative damage and its peroxidase activity is a plausible mechanism for cancer prevention by selenium. The GPX1 gene has a GCG repeat polymorphism in exon 1, coding for a polyalanine tract of five to seven alanine residues. To test if the GPX1 GCG repeat polymorphism associates with the risk of young-onset prostate cancer we conducted a case-control study. The GPX1Ala genotypes were determined for 267 prostate cancer cases and 260 control individuals using polymerase chain reaction (PCR) amplification with fluorescently labelled primers and an ABI 377 automated genotyper. Associations between specific genotypes and the risk of prostate cancer were examined by logistic regression. We found no significant association between the GPX1 genotypes and prostate cancer. There was however an increased frequency of the GPX1Ala6/Ala6 genotype in the prostate cancer cases compared to controls (OR: 1.67; 95% CI: 0.97-2.87). The result of this study suggests that the GPX1 genotype is unlikely to be associated with the risk of developing prostate cancer.
流行病学研究表明，低硒水平与前列腺癌的发生之间存在关联。人类细胞谷胱甘肽过氧化物酶I（hGPX1）是一种依赖硒的酶，可防止氧化损伤，其过氧化物酶活性是硒预防癌症的一种合理机制。GPX1基因在第1外显子中有一个GCG重复多态性，编码一个由5至7个丙氨酸残基组成的聚丙氨酸序列。为了测试GPX1 GCG重复多态性是否与年轻型前列腺癌的风险相关，我们进行了一项病例对照研究。使用荧光标记引物的聚合酶链反应（PCR）扩增和ABI 377自动基因分型仪，对267例前列腺癌病例和260名对照个体的GPX1Ala基因型进行了测定。通过逻辑回归分析特定基因型与前列腺癌风险之间的关联。我们发现GPX1基因型与前列腺癌之间没有显著关联。然而，与对照组相比，前列腺癌病例中GPX1Ala6/Ala6基因型的频率有所增加（比值比：1.67；95%置信区间：0.97 - 2.87）。这项研究的结果表明，GPX1基因型不太可能与前列腺癌的发生风险相关。
