Nakazawa I, Mead J F, Yonemoto R H
Lipids. 1976 Jan;11(1):79-82. doi: 10.1007/BF02532589.
The microsomal desaturase activity of human cancerous and noncancerous tissues was measured in vitro using 1-14C, -11, 14-eicosadienoic and 1-14C-oliec acids as substrates. Tissues used were a case of ovarian cancer, a urinary bladder cancer, a rectal cancer, and a non-specific colonic ulcer with appropriately normal tissues. When 11, 14-20:2 was used as substrate, radioactive tetraene and triene were produced. The tetraene was identified by radio gas chromatography as arachidonic acid (5, 8, 11, 14-20:4), and the triene had a retention time of 5, 11, 14-20:3. Thus, the possibility arises that a delta8 desaturase was involved. In the delta6 desaturase, with the urinary bladder cancerous tissue, the desaturase activity appeared to be decreased in comparison to neighboring tissue, whereas with the colonic cancer tissue, the desaturase appeared to be relatively increased, though the number of samples was inadequate for confidence.
以1-¹⁴C、-11,14-二十碳二烯酸和1-¹⁴C-油酸为底物，在体外测量了人类癌组织和非癌组织的微粒体去饱和酶活性。所用组织包括一例卵巢癌、一例膀胱癌、一例直肠癌以及伴有相应正常组织的非特异性结肠溃疡。当以11,14-20:2作为底物时，会产生放射性四烯和三烯。通过放射性气相色谱法鉴定，四烯为花生四烯酸（5,8,11,14-20:4），三烯的保留时间为5,11,14-20:3。因此，有可能涉及δ8去饱和酶。在δ6去饱和酶方面，与膀胱癌组织相邻的组织相比，膀胱癌组织中的去饱和酶活性似乎降低；而在结肠癌组织中，尽管样本数量不足以确定，但去饱和酶活性似乎相对增加。
