Borkotaky B, Bezbaruah R L
Biochemistry Division, Regional Research Laboratory, Jorhat 785 006, Assam, India.
Folia Microbiol (Praha). 2002;47(5):473-6. doi: 10.1007/BF02818783.
Asparaginase production by a mesophilic strain of Erwinia sp. was examined; the maximum of activity was found at 40 degrees C and pH 8.5. Among the various carbon sources, mannitol was shown to be the best for production of activity. Inorganic nitrogen sources were better than the organic ones. The enzyme activity was not inhibited by 10 mmol/L metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Co2+, Ni2+, Zn2+); the activity was strongly inhibited by addition of EDTA. L-Arginine, DL-alanine, L-asparagine and L-glutamine stimulated the L-asparaginase production by 3.9, 1.7, 4.3 and 4.0 fold, respectively. The combination of L-arginine, L-asparagine and L-glutamine synergistically stimulated the asparaginase up to 5.8 fold.
对嗜温欧文氏菌属菌株产生天冬酰胺酶的情况进行了研究；发现活性最大值出现在40摄氏度和pH值8.5时。在各种碳源中，甘露醇被证明是产生活性的最佳碳源。无机氮源优于有机氮源。10 mmol/L的金属离子（Na+、K+、Mg2+、Ca2+、Ba2+、Co2+、Ni2+、Zn2+）不会抑制该酶的活性；添加EDTA会强烈抑制该活性。L-精氨酸、DL-丙氨酸、L-天冬酰胺和L-谷氨酰胺分别使天冬酰胺酶的产量提高了3.9倍、1.7倍、4.3倍和4.0倍。L-精氨酸、L-天冬酰胺和L-谷氨酰胺的组合协同刺激天冬酰胺酶产量提高至5.8倍。
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