人生殖周期不同阶段输卵管素mRNA的可变表达。
Variable expression of oviductin mRNA at different stages of human reproductive cycle.
作者信息
Lok Ingrid H, Briton-Jones Christine M, Yuen Pong M, Haines Christopher J
机构信息
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, Hong Kong SAR, People's Republic of China.
出版信息
J Assist Reprod Genet. 2002 Dec;19(12):569-76. doi: 10.1023/a:1021263132176.
PURPOSE
To examine the in vivo expression of oviductin mRNA at different stages of the human female reproductive cycle including pregnancy and after menopause.
METHODS
Oviducts were obtained from 25 women in normal menstrual cycle, 5 in early pregnancy, 5 undergoing postpartum sterilization, and 4 menopausal women. The oviductal mucosal tissue was isolated and oviductin mRNA was assessed using reverse-transriptase-polymerase chain reaction (RT-PCR); its correlation with various hormones was assessed.
RESULTS
Oviductin mRNA was detected throughout the menstrual cycle, highest in the periovulatory period. It continued to be expressed in early pregnancy but was absent in the postpartum period and after menopause.
CONCLUSIONS
The production and function of oviductin at different stages of human reproductive cycle including pregnancy is not well known. Its highest expression at the time of ovulation is consistent with a supportive role in fertilization and early embryo development.
目的
研究输卵管蛋白mRNA在人类女性生殖周期不同阶段（包括孕期和绝经后）的体内表达情况。
方法
从25名处于正常月经周期的女性、5名早孕女性、5名接受产后绝育手术的女性以及4名绝经女性获取输卵管。分离输卵管黏膜组织，采用逆转录-聚合酶链反应（RT-PCR）评估输卵管蛋白mRNA；评估其与各种激素的相关性。
结果
在整个月经周期均检测到输卵管蛋白mRNA，在排卵期周围最高。在早孕期间它持续表达，但在产后及绝经后不存在。
结论
包括孕期在内的人类生殖周期不同阶段输卵管蛋白的产生和功能尚不清楚。其在排卵时的最高表达与在受精和早期胚胎发育中的支持作用一致。
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