使用液相色谱法同时测定人血浆中的九种抗逆转录病毒化合物。
Simultaneous determination of nine antiretroviral compounds in human plasma using liquid chromatography.
作者信息
Turner Michele L, Reed-Walker Kedria, King Jennifer R, Acosta Edward P
机构信息
Division of Clinical Pharmacology, University of Alabama at Birmingham, 1530 3rd Avenue South, VH 116, 35294-0019, Birmingham, AL, USA.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Feb 5;784(2):331-41. doi: 10.1016/s1570-0232(02)00822-x.
A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) method using UV detection was developed for the determination of nine antiretroviral compounds commonly found in plasma from patients receiving antiretroviral therapy. Analytes include indinavir, saquinavir, ritonavir, amprenavir, lopinavir, delavirdine, efavirenz, nelfinavir and its M8 metabolite. Analytes were isolated from plasma using tert.-butyl methyl ether and separation achieved via reversed-phase liquid chromatography on a C(8) column with a gradient mobile phase. Detection at 210 nm provided adequate sensitivity. Limit of quantification is 50 ng/ml and all analytes demonstrated linearity across 50-10000 ng/ml from a single 200-microliter plasma sample. Recovery from plasma was consistently high (>80%). This novel HPLC methodology allows us to simultaneously determine plasma concentrations of nine antiretrovirals, including lopinavir, in HIV-infected patients on a single HPLC system.
建立了一种使用紫外检测的快速、灵敏且特异的高效液相色谱（HPLC）方法，用于测定接受抗逆转录病毒治疗患者血浆中常见的九种抗逆转录病毒化合物。分析物包括茚地那韦、沙奎那韦、利托那韦、安普那韦、洛匹那韦、地拉韦啶、依非韦伦、奈非那韦及其M8代谢物。使用叔丁基甲醚从血浆中分离分析物，并通过在C(8)柱上的反相液相色谱，采用梯度流动相实现分离。在210nm处检测具有足够的灵敏度。定量限为50ng/ml，所有分析物在单个200微升血浆样品的50 - 10000ng/ml范围内均表现出线性。从血浆中的回收率始终很高（>80%）。这种新型HPLC方法使我们能够在单个HPLC系统上同时测定HIV感染患者血浆中包括洛匹那韦在内的九种抗逆转录病毒药物的浓度。
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