从土壤细菌在含有米糠和豆粕提取物的培养基中生产耐热耐酸植酸酶。
Thermo-acido-tolerant phytase production from a soil bacterium in a medium containing rice bran and soybean meal extract.
作者信息
Popanich Suthiluk, Klomsiri Chananart, Dharmsthiti Saovanee
机构信息
Centre for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Buddhamonthon 4 Rd., Salaya, Nakornpathom 73170, Thailand.
出版信息
Bioresour Technol. 2003 May;87(3):295-8. doi: 10.1016/s0960-8524(02)00236-5.
A bacterial strain capable of producing a thermo-acido-tolerant phytase was isolated from soil around haystacks and designated as strain PH01. The phytase produced was purified to homogeneity as determined by native PAGE. From SDS-PAGE, it was 30 kDa in size. The purified phytase was a thermo-acido-tolerant enzyme. A complex medium for the PH01 phytase production was developed. The medium, "PheB", was composed of 2% glucose, 0.2% CaCl(2), 0.5% NH(4)NO(3), 0.05% KCl, 0.05% MgSO(4).7H(2)O, 0.001% FeSO(4).7H(2)O, 0.001% MnSO(4).H(2)O in rice bran plus soybean meal extract containing 3% (v/v) phosphate solution (7.3% NaHPO(4)+3.2%KH(2)PO(4), pH 7.2). Cultivation was done at 37 degrees C with aeration for 48 h which produced phytase at 10 U/ml. Exposure of the phytase to 1% bile salt; i.e., taurocholate or deoxycholate, caused less than 15% reduction of activity. Potential application of PH01 phytase as a feed supplement was suggested.
从干草堆周围的土壤中分离出一株能够产生耐热嗜酸植酸酶的细菌菌株，命名为PH01菌株。通过非变性聚丙烯酰胺凝胶电泳（Native PAGE）测定，所产生的植酸酶被纯化至同质。根据十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE），其大小为30 kDa。纯化后的植酸酶是一种耐热嗜酸酶。开发了一种用于生产PH01植酸酶的复合培养基。该培养基“PheB”由2%葡萄糖、0.2%氯化钙（CaCl₂）、0.5%硝酸铵（NH₄NO₃）、0.05%氯化钾（KCl）、0.05%硫酸镁·7水合物（MgSO₄·7H₂O）、0.001%硫酸亚铁·7水合物（FeSO₄·7H₂O）、0.001%硫酸锰·水合物（MnSO₄·H₂O）组成，添加到含有3%（v/v）磷酸盐溶液（7.3%磷酸氢二钠（NaHPO₄）+3.2%磷酸二氢钾（KH₂PO₄），pH 7.2）的米糠加豆粕提取物中。在37℃通气培养48小时，产植酸酶量为10 U/ml。将植酸酶暴露于1%胆盐（即牛磺胆酸盐或脱氧胆酸盐）中，活性降低不到15%。建议将PH01植酸酶作为饲料添加剂进行潜在应用。
Zotero 插件