[CODIS基因座在排除父权中的应用研究]
[Study on application of CODIS loci to excluding paternity].
作者信息
Fang J X, Liu X J, Cheng D L
机构信息
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063.
出版信息
Fa Yi Xue Za Zhi. 2001 May;17(2):86-8.
OBJECTIVE
The study was carried out on application of CODIS loci (FGA, vWA, CSF1PO, TH01, TPOX, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11) in 100 cases of excluding paternity.
METHODS
The PCR amplified products of Profiler Plus and Cofiler amplification kit were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles.
RESULTS
In the group of Mother-Child-Alleged Father, more than 3 STR loci incompatibilities between alleged father and child were found in all observed cases, the mean incompatibility was 6.63; In the group of Alleged Father-Child, 94.0% of all cases observed was found more than 3 STR loci incompatibilities, the mean incompatibility was 5.01.
CONCLUSION
The results showed that CODIS loci had good application to excluding paternity, and the choice of hyperpolymorphic markers in investigation, which were valued by DP, H and PE, had direct relation with the augmentation of incompatibilities in excluding paternity.
目的
对100例排除父权案例应用CODIS基因座（FGA、vWA、CSF1PO、TH01、TPOX、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51和D21S11）进行研究。
方法
将Profiler Plus和Cofiler扩增试剂盒的PCR扩增产物注入ABI PRISM 310基因分析仪的毛细管中。GeneScan软件分析收集的数据，然后可将其导入Genotyper软件进行等位基因分型。
结果
在母子-被控父亲组中，所有观察案例均发现被控父亲与孩子之间存在3个以上STR基因座不相容，平均不相容数为6.63；在被控父亲-孩子组中，94.0%的观察案例发现存在3个以上STR基因座不相容，平均不相容数为5.01。
结论
结果表明，CODIS基因座在排除父权方面有良好应用，调查中选择受DP、H和PE评估的高多态性标记物与排除父权时不相容数的增加有直接关系。
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