[Design and synthesizing of human vascular endothelial growth factor (VEGF) peptide].

OBJECTIVE

To predict continuous epitopes of VEGF using synthetic peptide of VEGF as antigen to produce anti-VEGF-peptide monoclonal antibody.

METHODS

According to amino sequence of VEGF189, antigen index program and Goldkey soft were used to predict VEGF epitopes, and the peptide containing one of predicted epitopes was synthesized by 430A Peptide Synthesizer.

RESULTS

The NH2-terminal 1-26 amino acid sequence of VEGF was determined as synthetic peptide and synthesized.

CONCLUSION

The predicted epitope of VEGF was synthesized, which provided an effective method when lacking of VEGF.