美洲螯龙虾肝胰腺刷状缘膜囊泡（BBMV）对铁的摄取

Iron uptake by hepatopancreas brush border membrane vesicles (BBMV) of the lobster (Homarus americanus).

作者信息

Aslamkhan Amy G, Ahearn Gregory A

机构信息

Department of Physiology, University of Hawaii, Honolulu, Hawaii 96822, USA.

出版信息

J Exp Zool A Comp Exp Biol. 2003 Feb 1;295(2):145-50. doi: 10.1002/jez.a.10223.

DOI:10.1002/jez.a.10223

PMID:12541298

Abstract

The uptake of (55)Fe(2+) and solubilized (55)Fe(3+) into brush border membrane vesicles prepared from the hepatopancreas of the Atlantic lobster (Homarus americanus) was investigated. Non-specific surface binding of (55)Fe(2+) at equilibrium to the vesicular surface approximated 57% of total (55)Fe(2+) uptake. (55)Fe(2+) uptake showed temperature sensitivity and was trans-stimulated by a Ca(2+) gradient (at 5mM) directed out. Equilibrated (59)Fe(2+) exchanged for both Cd(2+) and cold Fe(2+). The data obtained in this study are suggestive that at least a portion of ferrous iron absorption may occur by a divalent exchanger mechanism.

摘要

研究了（55）铁（2+）和溶解的（55）铁（3+）被摄取进入由美洲龙虾（美洲螯龙虾）肝胰腺制备的刷状缘膜囊泡的情况。平衡时（55）铁（2+）在囊泡表面的非特异性表面结合量约占总（55）铁（2+）摄取量的57%。（55）铁（2+）的摄取表现出温度敏感性，并且受到向外的钙（2+）梯度（5毫摩尔）的转刺激。平衡的（59）铁（2+）可与镉（2+）和冷铁（2+）进行交换。本研究获得的数据表明，至少一部分亚铁的吸收可能通过二价交换机制发生。