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[高效液相色谱法测定伴有某些芳香物质的食品中的罂粟壳]

[Determination of Pericarpium papaveris in foods accompanied with some fragrant substances by high performance liquid chromatography].

作者信息

Dong N, Wang H Y

机构信息

Department of Chemistry, Guizhou University, Guiyang 550025, China.

出版信息

Se Pu. 2000 Nov;18(6):554-5.

Abstract

An HPLC method for the determination of Pericarpium papaveris in foods accompanied with some fragrant substances is described. The Pericarpium papaveris in food was extracted in alkaline condition with CHCl3-C2H5OH(9:1, V/V) and analyzed by reversed-phase HPLC by using a YWG C6H5 column and acetonitrile-methanol-water as mobile phase. The calibration curves of papaverine, morphine and codeine were linear in the range of 1.2 mg/L-100 mg/L and the mean recoveries were from 79.3% to 86.1%. The method has a good specificity and accuracy.

摘要

描述了一种用于测定食品中伴有某些芳香物质的罂粟壳的高效液相色谱法。食品中的罂粟壳在碱性条件下用三氯甲烷 - 乙醇(9:1,V/V)萃取,并用YWG C6H5柱,以乙腈 - 甲醇 - 水为流动相,通过反相高效液相色谱进行分析。罂粟碱、吗啡和可待因的校准曲线在1.2 mg/L - 100 mg/L范围内呈线性,平均回收率为79.3%至86.1%。该方法具有良好的特异性和准确性。

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