Sang J L, Wang Y C
Lab. of Cell Biology, Department of Biology, Beijing Normal University, Beijing 100875.
Shi Yan Sheng Wu Xue Bao. 1999 Dec;32(4):367-71.
Human breast cancer cell line Bcap-37 was stably transfected with the plasmid expressing antisense PKC alpha RNA, and cells, in which PKC alpha was inhibited due to antisense PKC alpha RNA, were isolated. Changes in serum-dependent growth in cell culture, cell clonogenicity in soft agar and growth in nude mice were tested, and the expressions of cyclin E and CDK2 were analyzed. After PKC alpha was inhibited, the cells showed that serum-dependent growth and anchorage-dependent growth enhanced, tumorigenicity in nude mice decreased. The results suggest that less aggressive breast cancer phenotypes may be induced by inhibition of PKC alpha. Levels of cyclin E and CDK2 mRNA in cells with antisense PKC alpha RNA were lower than those in control cell. These indicate that signal transduction system with PKC alpha is closely related to cell cycle control system with cyclin/CDK in the functions.
人乳腺癌细胞系Bcap-37用表达反义PKCαRNA的质粒进行稳定转染，然后分离出因反义PKCαRNA而使PKCα受到抑制的细胞。检测细胞培养中血清依赖性生长的变化、软琼脂中的细胞克隆形成能力以及裸鼠体内的生长情况，并分析细胞周期蛋白E和CDK2的表达。PKCα受到抑制后，细胞表现出血清依赖性生长和锚定依赖性生长增强，裸鼠体内的致瘤性降低。结果表明，抑制PKCα可能诱导侵袭性较低的乳腺癌表型。带有反义PKCαRNA的细胞中细胞周期蛋白E和CDK2 mRNA的水平低于对照细胞中的水平。这些表明，在功能上，PKCα信号转导系统与细胞周期蛋白/细胞周期蛋白依赖性激酶的细胞周期控制系统密切相关。
