Xiao C, Lu J, Tian X, Li X, Zhou P
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wei Sheng Wu Xue Bao. 2001 Oct;41(5):611-6.
A extracellular alkaline elastase producing bacterial strain Bacillus XE22-4-1, with the optimum pH of 10.0 for its growth, was isolated and screened from alkali lake of Tibet. 2% glucose and 0.25% yeast extract are respectively the appropriate carbon and nitrogen source on elastase production. Soymeal can promote elastase production. The fermentation conditions in a 2 L stirred tank were investigated. The results have revealed that the dissolved oxygen is the most effective factor on elastase production. The maximum elastolytic activity reached 266 u/mL among 48 hours of fermentation by combination of enhancing aeration and changing the mixing speed.
从西藏碱性湖泊中分离筛选出一株胞外碱性弹性蛋白酶产生菌芽孢杆菌XE22-4-1，其生长的最适pH为10.0。2%葡萄糖和0.25%酵母提取物分别是弹性蛋白酶产生的适宜碳源和氮源。豆粕可促进弹性蛋白酶的产生。研究了2 L搅拌罐中的发酵条件。结果表明，溶解氧是影响弹性蛋白酶产生的最有效因素。通过增强通气和改变搅拌速度相结合，在48小时发酵过程中，最大弹性水解活性达到266 u/mL。
