Suppr超能文献

S100A9独特的C末端参与花生四烯酸与异源复合物S100A8/A9结合的证据。

Evidence for the involvement of the unique C-tail of S100A9 in the binding of arachidonic acid to the heterocomplex S100A8/A9.

作者信息

Sopalla Claudia, Leukert Nadja, Sorg Clemens, Kerkhoff Claus

机构信息

Institute of Experimental Dermatology, University of Münster, von-Esmarch-Str. 58, D-48149 Münster, Germany.

出版信息

Biol Chem. 2002 Dec;383(12):1895-905. doi: 10.1515/BC.2002.213.

Abstract

Protein complexes formed by S100A8 and S100A9 represent the only AA-binding capacity in the human neutrophilic cytosol and are involved in the intracellular arachidonic acid metabolism. The formation of S100A8/A9 protein complexes and the binding of calcium to the complexes are prerequisites for the specific binding of polyunsaturated fatty acids. The present study was undertaken to characterize the fatty acid binding site within the protein complex. Deletions at both termini and point mutations of different basic amino acids especially within the extended C-terminal tail of human S100A9 were introduced. The S100A9 mutant proteins were then analyzed with respect to protein-protein interaction (GST pull down-assay and yeast two-hybrid system) and functional properties (arachidonic acid and calcium binding). The data give strong evidence that the unique C-tail of S100A9 containing the three consecutive histidine residues (His103-His105) represents the region to which the fatty acid carboxy-group is bound to the protein complex. The localization of the AA-binding site within the unique C-tail of S100A9 correlates with the fact that fatty acid binding has not yet been reported for other S100 proteins.

摘要

由S100A8和S100A9形成的蛋白质复合物代表了人类嗜中性粒细胞胞质溶胶中唯一的花生四烯酸结合能力,并参与细胞内花生四烯酸代谢。S100A8/A9蛋白质复合物的形成以及钙与该复合物的结合是多不饱和脂肪酸特异性结合的先决条件。本研究旨在表征蛋白质复合物内的脂肪酸结合位点。对人S100A9的两个末端进行了缺失突变,并对不同碱性氨基酸进行了点突变,特别是在其延长的C末端尾巴内。然后对S100A9突变蛋白进行了蛋白质-蛋白质相互作用分析(GST下拉分析和酵母双杂交系统)以及功能特性分析(花生四烯酸和钙结合)。数据提供了强有力的证据,表明S100A9独特的C末端尾巴包含三个连续的组氨酸残基(His103-His105),该区域是脂肪酸羧基与蛋白质复合物结合的部位。S100A9独特C末端尾巴内花生四烯酸结合位点的定位与以下事实相关:尚未有其他S100蛋白具有脂肪酸结合的报道。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验