Pabón Adriana, Carmona Jaime, Burgos Luis C, Blair Silvia
Grupo Malaria, Universidad de Antioquia, Medellín-Colombia, Colombia.
Clin Biochem. 2003 Feb;36(1):71-8. doi: 10.1016/s0009-9120(02)00423-x.
To compare oxidative stress in adults with non-complicated malaria and healthy controls.
We measured malondialdehyde (MDA), total antioxidant status (TAS), catalase, superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX). Oxidative stress was calculated based on MDA/TAS, MDA/GSH-PX and SOD/catalase indexes.
Mean MDA in patients was 3.9 micromol/L (controls = 1.3 micromol/L). Mean TAS was 0.9 mmol/L in patients and controls. Malaria patients had less catalase activity when compared to controls (209.4 vs. 320.4 k/gr), while SOD and GSH-PX activity was higher (79.4 U/mL, 11,884.2U/L vs. 54.3 U/mL, 9,672.6 U/L). MDA/TAS index was 3.5 fold more in patients than in controls, MDA/GSH-PX and SOD/catalase indexes were increased by 6 and 2.8 fold. MDA levels and MDA/TAS index showed no differences according to malarial history, parasitaemia, Plasmodium species, parasite's stage, place of residence and drinking or smoking habits.
During acute non-complicated P. falciparum or P. vivax malaria, we observed high oxidative stress. This resulted from lipid peroxidation rather than from a reduced TAS. We propose MDA/TAS index as a useful marker of oxidative stress during malaria infection.
比较非复杂性疟疾成人患者与健康对照者的氧化应激情况。
我们测量了丙二醛（MDA）、总抗氧化状态（TAS）、过氧化氢酶、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）。基于MDA/TAS、MDA/GSH-PX和SOD/过氧化氢酶指数计算氧化应激。
患者的平均MDA为3.9微摩尔/升（对照组为1.3微摩尔/升）。患者和对照组的平均TAS均为0.9毫摩尔/升。与对照组相比，疟疾患者的过氧化氢酶活性较低（209.4对320.4 k/gr），而SOD和GSH-PX活性较高（79.4 U/mL，11,884.2U/L对54.3 U/mL，9,672.6 U/L）。患者的MDA/TAS指数比对照组高3.5倍，MDA/GSH-PX和SOD/过氧化氢酶指数分别增加了6倍和2.8倍。MDA水平和MDA/TAS指数在疟疾病史、寄生虫血症、疟原虫种类、寄生虫阶段、居住地点以及饮酒或吸烟习惯方面均无差异。
在急性非复杂性恶性疟或间日疟期间，我们观察到高氧化应激。这是由脂质过氧化引起的，而非TAS降低所致。我们建议将MDA/TAS指数作为疟疾感染期间氧化应激的有用标志物。
