Nelson D L, Molinoff P B
J Pharmacol Exp Ther. 1976 Feb;196(2):346-59.
The subcellular distribution of adrenergic storage vesicles was determined in rat spleen, heart and superior cervical ganglion and in dog spleen using dopamine-beta-hydroxylase (DBH) as a marker for vesicle membranes and norepinephrine (NE) as a marker for the soluble contents of the storage vesicles. Density gradient centrifugation revealed populations of vesicles in the rat heart and spleen with equilibrium densities of 1.05 and 1.15 g/ml. There was a consistent shoulder on the low-density peak at a density of 1.08 g/ml. Several populations of NE-and DBH-containing vesicles were also found in homogenates of dog spleen. In homogenates of superior cervical ganglia, most of the NE was nonsedimentable while the DBH activity formed a broad poorly resolved band within the gradient. Although NE and DBH were present in each of several populations of vesicles in nerve terminals of rat and dog organs, the ratio of NE/DBH varied as a function of the species and organ examined. Mitochondrial contamination did not, as has been previously claimed, influence the distribution of DBH which was observed. Both light and heavy vesicles were able to take up and retain NE-[3H].
利用多巴胺-β-羟化酶（DBH）作为囊泡膜的标志物，去甲肾上腺素（NE）作为储存囊泡可溶性内容物的标志物，测定了大鼠脾脏、心脏和颈上神经节以及犬脾脏中肾上腺素能储存囊泡的亚细胞分布。密度梯度离心显示，大鼠心脏和脾脏中存在平衡密度分别为1.05和1.15 g/ml的囊泡群体。在密度为1.08 g/ml的低密度峰处有一个一致的肩部。在犬脾脏匀浆中也发现了几个含有NE和DBH的囊泡群体。在颈上神经节匀浆中，大部分NE不可沉降，而DBH活性在梯度内形成一条宽且分辨率差的带。尽管在大鼠和犬器官的神经末梢中，几个囊泡群体中都存在NE和DBH，但NE/DBH的比值因所检查的物种和器官而异。线粒体污染并未如先前所说的那样影响所观察到的DBH分布。轻囊泡和重囊泡都能够摄取并保留NE-[3H]。
