[香蕉束顶病植原体16S rDNA的扩增、16S rDNA片段的限制性片段长度多态性分析及序列分析]
[Amplification of phytoplasma 16S rDNA from banana bunchy top disease, RFLP and sequence analysis of 16S rDNA fragment].
作者信息
Li H, Qiu B, Li L, Shi C
机构信息
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
出版信息
Wei Sheng Wu Xue Bao. 1999 Aug;39(4):315-20.
PMID:12555569
Abstract
Extract DNA from banana plants with banana bunchy top disease and amplify the phytoplasma 16S rDNA fragment by nested PCR. The classification of the phytoplasma established on the basis of 16S rDNA restriction patterns was examined by performing a sequence analysis of 16S rDNA fragment. The RFLP pattern, sequence data and phylogenic tree showed that this phytoplasma should belong to Group I(Phytoplasma asteri).
摘要
从患有香蕉束顶病的香蕉植株中提取DNA，并通过巢式PCR扩增植原体16S rDNA片段。通过对16S rDNA片段进行序列分析，研究基于16S rDNA限制性图谱建立的植原体分类。RFLP图谱、序列数据和系统发育树表明，该植原体应属于I组（翠菊植原体）。
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