Feeney Susan A, Simpson David A C, Gardiner Thomas A, Boyle Cliona, Jamison Pamela, Stitt Alan W
Department of Ophthalmology, The Queen's University of Belfast, The Royal Victoria Hospital, Belfast, Northern Ireland, United Kingdom.
Invest Ophthalmol Vis Sci. 2003 Feb;44(2):839-47. doi: 10.1167/iovs.02-0040.
Vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF) are members of a large group of homologous peptides identified as the VEGF family. Although VEGF-A is known to act as a potent angiogenic peptide in the retina, the vasoactive function of PlGF in this tissue is less well defined. This study has sought to elucidate the expression patterns and modulatory role of these growth factors during retinal vascular development and hyaloid regression in the neonatal mouse.
C57BL6J mice were killed at postnatal days (P)1, P3, P5, P7, P9, and P11. The eyes were enucleated and processed for in situ hybridization and immunocytochemistry and the retinas extracted for total protein or RNA. Separate groups of neonatal mice were also injected intraperitoneally daily from P2 through P9 with either VEGF-neutralizing antibody, PlGF-neutralizing antibody, isotype immunoglobulin (Ig)-G, or phosphate-buffered saline (PBS). The mice were then perfused with fluorescein isothiocyanate (FITC)-dextran, and the eyes were subsequently embedded in paraffin wax or flat mounted.
Quantitative (real-time) reverse transcription-polymerase chain reaction (RT-PCR) demonstrated similar expression patterns of VEGF-A and PlGF mRNA during neonatal retinal development, although the fluctuation between time periods was greater overall for VEGF-A. The localization of VEGF-A and PlGF in the retina, as revealed by in situ hybridization and immunohistochemistry, was also similar. Neutralization of VEGF-A caused a significant reduction in the hyaloid and retinal vasculature, whereas PlGF antibody treatment caused a marked persistence of the hyaloid without significantly affecting retinal vascular development.
Although having similar expression patterns in the retina, these growth factors appear to have distinct modulatory influences during normal retinal vascular development and hyaloid regression.
血管内皮生长因子（VEGF）-A和胎盘生长因子（PlGF）是被鉴定为VEGF家族的一大组同源肽成员。虽然已知VEGF-A在视网膜中作为一种强效血管生成肽发挥作用，但PlGF在该组织中的血管活性功能尚不明确。本研究旨在阐明这些生长因子在新生小鼠视网膜血管发育和玻璃体退化过程中的表达模式及调节作用。
在出生后第（P）1、3、5、7、9和11天处死C57BL6J小鼠。摘除眼球，进行原位杂交和免疫细胞化学处理，并提取视网膜用于总蛋白或RNA检测。从出生后第2天至第9天，还分别每天给新生小鼠腹腔注射VEGF中和抗体、PlGF中和抗体、同型免疫球蛋白（Ig）-G或磷酸盐缓冲盐水（PBS）。然后用异硫氰酸荧光素（FITC）-葡聚糖灌注小鼠，随后将眼球包埋在石蜡中或进行平铺制片。
定量（实时）逆转录-聚合酶链反应（RT-PCR）显示，在新生视网膜发育过程中，VEGF-A和PlGF mRNA的表达模式相似，尽管VEGF-A在各时间段之间的波动总体上更大。原位杂交和免疫组织化学显示，VEGF-A和PlGF在视网膜中的定位也相似。VEGF-A的中和导致玻璃体和视网膜血管显著减少，而PlGF抗体处理导致玻璃体明显持续存在，且对视网膜血管发育无显著影响。
尽管这些生长因子在视网膜中具有相似的表达模式，但在正常视网膜血管发育和玻璃体退化过程中，它们似乎具有不同的调节作用。
Zotero 插件