石斑鱼β-诺达病毒衣壳蛋白末端在病毒样颗粒组装中的作用。

Involvement of the terminus of grouper betanodavirus capsid protein in virus-like particle assembly.

作者信息

Lu M W, Lin C S

机构信息

Institute of Marine Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan.

出版信息

Arch Virol. 2003 Feb;148(2):345-55. doi: 10.1007/s00705-002-0901-8.

DOI:10.1007/s00705-002-0901-8

PMID:12556997

Abstract

Dragon grouper, Epinephelus lanceolatus, nervous necrosis virus (DGNNV) consists of 180 copies of capsid protein that encapsulates a bipartite genome of single-stranded (+)RNAs. Expressing the open reading frame (ORF) of RNA2 in Escherichia coli forms virus-like particles (VLPs) that resembles native virus. Deleting N- and C-termini revealed different impacts on VLP formation. Deletion of 35 or 52 residues at the N-terminus completely ruined the VLP assembly, presumably due to removal of positively charged residues for binding RNAs. When deletions were restricted to 4, 16, or 25 N-terminal residues, the assembly of VLPs remained. The ability of VLP formation diminished when 4 to 11 C-terminal residues were deleted. The termini that can be deleted without seriously destructing the VLPs are 25 and 3 residues at N- and C-termini, respectively.

摘要

波纹唇鱼神经坏死病毒（DGNNV）由180个衣壳蛋白拷贝组成，这些衣壳蛋白包裹着一个双链单链（+）RNA基因组。在大肠杆菌中表达RNA2的开放阅读框（ORF）会形成类似于天然病毒的病毒样颗粒（VLP）。删除N端和C端对VLP形成有不同影响。在N端删除35或52个残基会完全破坏VLP组装，可能是由于去除了用于结合RNA的带正电荷残基。当删除限制在4、16或25个N端残基时，VLP的组装仍然存在。当删除4至11个C端残基时，VLP形成能力下降。在不严重破坏VLP的情况下可以删除的末端分别是N端的25个残基和C端的3个残基。