[抗肠出血性大肠杆菌O抗原单克隆抗体的制备与鉴定]
[Production and characterization of monoclonal antibody against the O antigen of Enterohemorrhagic Escherichia coli].
作者信息
Zhao Zhijing, Liu Xiumei
机构信息
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
出版信息
Wei Sheng Yan Jiu. 2002 Apr;31(2):108-11.
Spleen cells collected from Balb/c mice immunized with the somatic antigen of Enterohaemorrhagic E. coli O157:H7 are fused with murine Sp2/0 myeloma cells. A hybridoma cell line specific to E. coli O157 is established after screening and sub-cloning and is designated as 3A5. It belongs to a subtype of IgM and the ascetic titer of the antibody is 1:1 x 10(6). The detection limit is 10(5)-10(6) cfu/ml. This monoclonal antibody(McAb) also has activity with Enterohaemorrhagic E. coli O113:H21.
从用肠出血性大肠杆菌O157:H7体细胞抗原免疫的Balb/c小鼠中收集脾细胞，与小鼠Sp2/0骨髓瘤细胞融合。经筛选和亚克隆后建立了一种对大肠杆菌O157特异的杂交瘤细胞系，命名为3A5。它属于IgM亚型，抗体腹水效价为1:1×10(6)。检测限为10(5)-10(6) cfu/ml。该单克隆抗体（McAb）对肠出血性大肠杆菌O113:H21也有活性。
Zotero 插件