[酶联免疫吸附测定法测定赭曲霉毒素A Ⅰ. 赭曲霉毒素A抗原制备的研究]

[Determination of ochratoxin A by ELISA I. Study on the preparation of ochratoxin a antigen].

作者信息

Chen Xuelan, Xu Yang, Wu Chenggang

机构信息

Sino-German Joint Research Institute, Nanchang 330047, China.

出版信息

Wei Sheng Yan Jiu. 2002 Feb;31(1):53-4, backcover.

PMID:12561576

Abstract

Ochratoxin A (OTA) antigen was prepared by activated ester method. Factors influencing OTA antigen were discussed and the optimum conditions was found by L9(3(4)) orthogonal design. The results showed that when the mole ratio was OTA:BSA = 20:1, OTA:NHS(N-hydroxy-succinamide):DCC(dicyclohexylcarbodiimide) = 1:2:4, the activate time was 120 min and the conjugation time was 90 min, the utilization of OTA could reach 48.2% and the best conjugation ratio of OTA and BSA was 9.64.

摘要

采用活化酯法制备赭曲霉毒素A（OTA）抗原。探讨了影响OTA抗原的因素，并通过L9(3(4))正交设计找到了最佳条件。结果表明，当摩尔比为OTA:BSA = 20:1、OTA:NHS（N-羟基琥珀酰亚胺）:DCC（二环己基碳二亚胺） = 1:2:4，活化时间为120分钟，偶联时间为90分钟时，OTA的利用率可达48.2%，OTA与BSA的最佳偶联比为9.64。