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酶联免疫吸附测定试剂盒在山羊普氏原羚和骆驼纹背颈龟血清学诊断中皮下病的评估。

Evaluation of ELISA kit hypodermosis in serodiagnosis of Przhevalskiana silenus in goats and Cephalopenia titillator in camels.

作者信息

Morsy T A, Farrag A M, Mazyad S A, Abou-Gamra M M

机构信息

Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.

出版信息

J Egypt Soc Parasitol. 1999;29(3):709-19.

Abstract

Myiasis is a problem of Medical and Veterinary importance worldwide. It affects human welfare both directly and indirectly. ELISA kit hypodermosis was evaluated in detecting antibodies against two oestrid larvae, Przhevalskiana silenus in goats and Cephalopina titillator in camels. Both animals were parasitologically proved to have oestrid myiasis infestation. Antibodies against P. silenus were detected in 38 out of 40 (95%) infested goats and negative results among the control group. With C. titillator, antibodies were detected in 39 out of 40 (97.5%) infested camels. But one of the control (10%) gave false seropositivity. ELISA kit hypodermosis diagnosed antibodies against P. silenus in goats and C. titillator in camels. Consequently, there are cross reactions between anti-hypoderma antibodies and antibodies against both Przhevalskiana and Cephalopina. ELISA is confirmed to be an easy and economic tool in diagnosing myiasis infestation when direct examination is not a simple mean and/or for epidemiological studies. The whole results were discussed. Focusing on the clinical pictures and pathogenesis of human myiasis was included to clarify the zoonotic importance of myiasis in Egypt.

摘要

蝇蛆病是一个在全球范围内具有医学和兽医学重要性的问题。它直接或间接地影响人类福祉。对用于检测山羊体内针对两种狂蝇幼虫(普氏狂蝇)和骆驼体内针对羊狂蝇抗体的酶联免疫吸附测定(ELISA)皮下包虫病试剂盒进行了评估。经寄生虫学证实,这两种动物均感染了狂蝇蛆病。在40只受感染的山羊中,有38只(95%)检测到针对普氏狂蝇的抗体,而对照组结果为阴性。对于羊狂蝇,在40只受感染的骆驼中有39只(97.5%)检测到抗体。但有一只对照骆驼(10%)出现假血清阳性。ELISA皮下包虫病试剂盒诊断出山羊体内针对普氏狂蝇和骆驼体内针对羊狂蝇的抗体。因此,抗皮下包虫抗体与针对普氏狂蝇和羊狂蝇的抗体之间存在交叉反应。当直接检查并非简便方法和/或用于流行病学研究时,ELISA被证实是诊断蝇蛆病感染的一种简便且经济的工具。对整个结果进行了讨论。其中包括关注人类蝇蛆病的临床症状和发病机制,以阐明埃及蝇蛆病的人畜共患病重要性。

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