[曼氏血吸虫两种易化葡萄糖转运蛋白在日本血吸虫体表的免疫细胞化学定位]
[Immunocytochemical localization of two facilitated glucose transporters of Schistosoma mansoni in the tegument of Schistosoma japonicum].
作者信息
Jiang J, Zhong C, Qi L, Yu Y
机构信息
Department of Biophsics, Shanghai Medical University, Shanghai 200032.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):222-4.
AIM
To observe the distribution of Schistosoma mansoni glucose transport proteins, SGTP1 and SGTP4, in the tegument of Schistosoma japonicum.
METHODS
The rapidly frozen fixation technique and ultracryomicrotomy were adopted for preparing ultrathin cryosections of S. japonicum. Anti-SGTP1 and anti-SGTP4 antibodies were used to localize the corresponding antigens in the tegument of adult S. japonicum by immunocytochemical technique.
RESULTS
SGTP1 was localized on the basal membrane of the tegument and its infoldings, SGTP4 was localized on the apical membrane of the tegument and its invaginations of S. japonicum.
CONCLUSION
The same localization for SGTP 1 and SGTP4 in the tegument of S. japonicum and S. mansoni exhibited apparent homology between SGTPs of the two schistosomes.
目的
观察曼氏血吸虫葡萄糖转运蛋白SGTP1和SGTP4在日本血吸虫体表的分布情况。
方法
采用快速冷冻固定技术和超薄冷冻切片技术制备日本血吸虫超薄冰冻切片。运用免疫细胞化学技术，使用抗SGTP1和抗SGTP4抗体在日本血吸虫成虫体表定位相应抗原。
结果
SGTP1定位于日本血吸虫体表基底膜及其内褶处，SGTP4定位于日本血吸虫体表顶端膜及其内陷处。
结论
SGTP1和SGTP4在日本血吸虫体表的定位相同，且两种血吸虫的SGTPs之间存在明显同源性。
Zotero 插件