[微小隐孢子虫DNA探针的制备]
[Preparation of DNA probe for Cryptosporidium parvum].
作者信息
Guo B, Zhang J, Lian D
机构信息
Department of parasitology, Chagzhi Medical College, Changzhi 046000.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):113-4.
AIM
To prepare a probe with high specificity and sensitivity for the detection of Cryptosporidium parvum.
METHODS
Using PCR method, a fragment from the DNA of C. parvum was amplified. The PCR product, 452 bp DNA, was labeled with hapten digoxigenin.
RESULTS
Examination of sensitivity showed that the DNA probe could detect as low as 2 pg DNA from C. parvum. The dot-blot hybridizition assay showed that the probe hybridized with the DNA of C. parvum, but not hybridized with DNA of E. histolytica, G. lamblia, E. coli and D. bacilli.
CONCLUSION
The probe was highly specific and sensitive for the detection of C. parvum.
目的
制备一种用于检测微小隐孢子虫的具有高特异性和敏感性的探针。
方法
采用聚合酶链反应（PCR）方法，扩增微小隐孢子虫DNA的一个片段。PCR产物，即452 bp的DNA，用半抗原地高辛标记。
结果
敏感性检测表明，该DNA探针能检测到低至2 pg的微小隐孢子虫DNA。斑点杂交试验表明，该探针能与微小隐孢子虫的DNA杂交，但不与溶组织内阿米巴、蓝氏贾第鞭毛虫、大肠杆菌和双歧杆菌的DNA杂交。
结论
该探针在检测微小隐孢子虫方面具有高度的特异性和敏感性。
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