微波辐射酶联免疫吸附测定法诊断日本血吸虫病的初步研究

[Preliminary studies on microwave irradiation ELISA for diagnosis of Schistosomiasis japonica].

作者信息

Gu Q, Zhang H, Zhu S, Xu W

机构信息

Hangzhou Medical College, Hangzhou 310012.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):115-6.

PMID:12563796

Abstract

AIM

To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica.

METHODS

Using microwave irradiation ELISA (MWI-ELISA) and fast-ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases.

RESULTS

The positive rates of schistosomiasis cases were 88.1% (104/118) by MWI-ELISA and 91.5% (108/118) by fast-ELISA, respectively (chi 2 = 0.74, P > 0.05). The false positive reaction of healthy individuals was found in 2 cases(3.3%) by MWI-ELISA and 1 case (1.6%) by fast-ELISA, respectively (chi 2 = 0.34, P > 0.05). No false positive reaction was found in paragonimiasis cases.

CONCLUSION

The sensitivity and specificity of the two tests were similar, however, MWI-ELISA was faster than fast-ELISA.

摘要

目的

探索一种快速高效的日本血吸虫病诊断方法。

方法

采用微波辐射酶联免疫吸附试验（MWI-ELISA）和快速酶联免疫吸附试验检测118例日本血吸虫病患者、61例健康个体及12例肺吸虫病患者血清中的特异性抗体。

结果

MWI-ELISA检测日本血吸虫病患者的阳性率为88.1%（104/118），快速酶联免疫吸附试验的阳性率为91.5%（108/118）（χ² = 0.74，P > 0.05）。MWI-ELISA检测健康个体出现2例假阳性反应（3.3%），快速酶联免疫吸附试验出现1例假阳性反应（1.6%）（χ² = 0.34，P > 0.05）。肺吸虫病患者未出现假阳性反应。