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微波辐射酶联免疫吸附测定法诊断日本血吸虫病的初步研究

[Preliminary studies on microwave irradiation ELISA for diagnosis of Schistosomiasis japonica].

作者信息

Gu Q, Zhang H, Zhu S, Xu W

机构信息

Hangzhou Medical College, Hangzhou 310012.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):115-6.

PMID:12563796
Abstract

AIM

To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica.

METHODS

Using microwave irradiation ELISA (MWI-ELISA) and fast-ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases.

RESULTS

The positive rates of schistosomiasis cases were 88.1% (104/118) by MWI-ELISA and 91.5% (108/118) by fast-ELISA, respectively (chi 2 = 0.74, P > 0.05). The false positive reaction of healthy individuals was found in 2 cases(3.3%) by MWI-ELISA and 1 case (1.6%) by fast-ELISA, respectively (chi 2 = 0.34, P > 0.05). No false positive reaction was found in paragonimiasis cases.

CONCLUSION

The sensitivity and specificity of the two tests were similar, however, MWI-ELISA was faster than fast-ELISA.

摘要

目的

探索一种快速高效的日本血吸虫病诊断方法。

方法

采用微波辐射酶联免疫吸附试验(MWI-ELISA)和快速酶联免疫吸附试验检测118例日本血吸虫病患者、61例健康个体及12例肺吸虫病患者血清中的特异性抗体。

结果

MWI-ELISA检测日本血吸虫病患者的阳性率为88.1%(104/118),快速酶联免疫吸附试验的阳性率为91.5%(108/118)(χ² = 0.74,P > 0.05)。MWI-ELISA检测健康个体出现2例假阳性反应(3.3%),快速酶联免疫吸附试验出现1例假阳性反应(1.6%)(χ² = 0.34,P > 0.05)。肺吸虫病患者未出现假阳性反应。

结论

两种检测方法的敏感性和特异性相似,但MWI-ELISA比快速酶联免疫吸附试验速度更快。

相似文献

1
[Preliminary studies on microwave irradiation ELISA for diagnosis of Schistosomiasis japonica].微波辐射酶联免疫吸附测定法诊断日本血吸虫病的初步研究
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):115-6.
2
[Application of microwave irradiating ELISA for serological detection of schistosomiasis japonica].[微波辐照酶联免疫吸附测定法在日本血吸虫病血清学检测中的应用]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(1):54.
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[Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].[用高碘酸盐处理的可溶性虫卵抗原酶联免疫吸附测定法诊断血吸虫病的研究]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(4):238-41.
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[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies].用多克隆抗体和单克隆抗体的夹心酶联免疫吸附测定法检测日本血吸虫病患者循环抗原
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(2):92-3.
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[Evaluation of Schistosoma japonicum tegumental membrane antigen (SjMAg) in immunodiagnosis and efficacy assessment].日本血吸虫体表膜抗原(SjMAg)在免疫诊断及疗效评估中的应用
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(2):69-72.
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[Detection of different class (subclass) antibodies in sera of patients with schistosomiasis japonica for diagnosis and efficiency evaluation].
Hunan Yi Ke Da Xue Xue Bao. 1997;22(5):381-3.
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[Comparison of circulating antigen detection in schistosomiasis japonica patients using two methods of ELISA].[两种酶联免疫吸附测定法检测日本血吸虫病患者循环抗原的比较]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(4):227-8.
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[Preparation and diagnostic application of monoclonal antibodies against Schistosoma japonicum].抗日本血吸虫单克隆抗体的制备及诊断应用
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1991;9(4):254-7.
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[Antibody detection in sera of patients with Schistosomiasis japonica by dot immunogold filtration assay].[应用斑点免疫金渗滤法检测日本血吸虫病患者血清抗体]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Apr 30;24(2):146-7, 155.
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[The diagnostic value of combined detection of circulating antigens and antibodies in urine of patients with schistosomiasis japonica].[日本血吸虫病患者尿液中循环抗原与抗体联合检测的诊断价值]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(1):24-5.

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