[4-氨基吡啶对豚鼠心室肌细胞钙电流和钠电流的影响]
[Effects of 4-aminopyridine on calcium currents and sodium currents in guinea pig ventricular myocytes].
作者信息
Fu L Y, Li Y, Xia G J, Yao W X, Jiang M X
机构信息
Department of Pharmacology, School of Basic Medical Science, Tongji Medical University, Wuhan 430030, China.
出版信息
Yao Xue Xue Bao. 2001 Apr;36(4):250-3.
AIM
To investigate the effect of 4-aminopyridine (4-AP) on ion channels of myocytes.
METHODS
L-type calcium channel and sodium channel currents were recorded in guinea pig single ventricular myocyte using whole-cell patch-clamp techniques.
RESULTS
4-AP, 0.1, 0.5 and 1.0 mmol.L-1 were shown to inhibit L-type calcium channel currents (ICa, L) and sodium channel currents (INa) concentration-dependently. The percentage of inhibition were (11.6 +/- 1.7)%, (37.5 +/- 8.3)% and (54.5 +/- 6.9)% (P < 0.01) respectively for ICa, L, and (22.1 +/- 14.3)% (P < 0.05), (39.4 +/- 8.8)% and (62.3 +/- 6.8)% (P < 0.01) respectively for INa. 4-AP 0.5 mmol.L-1 shifted the I-V curves of ICa, L and INa upwardly.
CONCLUSION
4-AP blocked L-type calcium channel and sodium channels in guinea-pig ventricular myocytes concentration-dependently.
目的
研究4-氨基吡啶（4-AP）对心肌细胞离子通道的影响。
方法
采用全细胞膜片钳技术记录豚鼠单个心室肌细胞的L型钙通道和钠通道电流。
结果
0.1、0.5和1.0 mmol.L-1的4-AP呈浓度依赖性抑制L型钙通道电流（ICa,L）和钠通道电流（INa）。ICa,L的抑制百分比分别为（11.6±1.7）%、（37.5±8.3）%和（54.5±6.9）%（P<0.01），INa的抑制百分比分别为（22.1±14.3）%（P<0.05）、（39.4±8.8）%和（62.3±6.8）%（P<0.01）。0.5 mmol.L-1的4-AP使ICa,L和INa的I-V曲线向上移位。
结论
4-AP对豚鼠心室肌细胞的L型钙通道和钠通道具有浓度依赖性阻断作用。
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