前胡丙素对肾血管性高血压大鼠和自发性高血压大鼠血管肥厚、[Ca2+]i、胶原含量及一氧化氮的影响
[Effects of praeruptorin C on vascular hypertrophy, [Ca2+]i, collagen content and NO in renovascular and spontaneously hypertensive rats].
作者信息
Rao M R, Liu W B, Liu P Q
机构信息
Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China.
出版信息
Yao Xue Xue Bao. 2001 Mar;36(3):165-9.
AIM
To study the effects of praeruptorin C (pra-C), a pure constituent isolated from "Qian-Hu", the roots of Peucedanum praeruptorum Dunn. (Umbelliferae), on vascular hypertrophy, collagen content, transient [Ca2+]i, NO and vascular response of the thoracic aorta of renovascular and spontaneously hypertensive rats (RHR, SHR).
METHODS
RHR and SHR were given pra-C 20 mg.kg-1.d-1 for 9 weeks, ig. Blood pressure of both rats were measured using tail cuff manometry. Under inverted microscopy the length and width of the smooth muscle cells were measured by using computer software MICC (Dongnan University). [Ca2+]i of smooth muscle cell (SMCs) was measured with Fura-2/AM. By measuring the specific aminoacid hydroxyproline content, the collagen content was obtained. By using Griess reagent, the NO in the smooth muscle cells (SMCs) was measured.
RESULTS
The intermedia of the thoracic aorta in RHR was enlarged than that of the normal and pra-C groups. The size (length x width) of the SMCs of thoracic aorta from RHR increased 73.4 microns vs nomal 34.5 microns and pra-C 34 microns. The collagen content of thoracic aorta was 39% +/- 6.8% dry weight in RHR, they were 26.5% +/- 3% dry weight in normal and 25.6% +/- 1.1% dry weight in pra-C, RHR vs pra-C. The resting [Ca2+]i of single cell of SMCs was (62 +/- 6) nmol.L-1. In Hanks solution containing CaCl2 1 mmol.L-1, the resting [Ca2+]i of SMCs was (150 +/- 8) nmol.L-1 in normal. (226 +/- 11) nmol.L-1 in RHR. In presence of KCl 60 mmol.L-1, NE 10 mumol.L-1, ANG II 100 nmol.L-1 and ATP 30 mumol.L-1 the [Ca2+]i of SMCs were increased by 128%; 132%; 233% and 152% in RHR, respectively. The pra-C group was similar to the normal group. The resting [Ca2+]i of SMCs was (71 +/- 6) nmol.L-1 in control of SHR, in Hanks solution containing CaCl2 1 mmol.L-1. The resting [Ca2+]i of SMCs was (160 +/- 8) nmol.L-1 in normal, and (362 +/- 18) nmol.L-1 in SHR. In presence KCl 60 mmol.L-1 and NE 10 mumol.L-1 the [Ca2+]i of SMCs were increased by 235% and 200% in SHR, respectively. Pra-C group was similar to normal group. NO of SMCs was decreased 76% in SHR, pra-C group was nearly normal. The pra-C improved vascular responses of the thoracic aorta of RHR.
CONCLUSION
These results indicate that pra-C improved the vascular hypertrophy by decreasing the size of SMCs cells, collagen content. SMCs [Ca2+]i and increasing NO production.
目的
研究从伞形科植物白花前胡（Peucedanum praeruptorum Dunn.）根中分离得到的一种纯成分白花前胡丙素（pra-C）对肾血管性高血压大鼠（RHR）和自发性高血压大鼠（SHR）胸主动脉血管肥厚、胶原含量、瞬时[Ca2+]i、一氧化氮（NO）及血管反应的影响。
方法
RHR和SHR均按20mg·kg-1·d-1ig给予pra-C，持续9周。用尾套法测量两组大鼠血压。在倒置显微镜下，使用东南大学的计算机软件MICC测量平滑肌细胞的长度和宽度。用Fura-2/AM测量平滑肌细胞（SMCs）的[Ca2+]i。通过测量特定氨基酸羟脯氨酸含量得出胶原含量。用Griess试剂测量平滑肌细胞中的NO。
结果
RHR胸主动脉中膜比正常组和pra-C组增厚。RHR胸主动脉SMCs的大小（长×宽）增加至73.4微米，正常组为34.5微米，pra-C组为34微米。RHR胸主动脉胶原含量为干重的39%±6.8%，正常组为26.5%±3%，pra-C组为25.6%±1.1%，RHR与pra-C组相比。单个SMCs的静息[Ca2+]i为（62±6）nmol·L-1。在含1mmol·L-1CaCl2的Hanks溶液中，正常组SMCs的静息[Ca2+]i为（150±8）nmol·L-1，RHR为（226±11）nmol·L-1。在60mmol·L-1KCl、10μmol·L-1去甲肾上腺素（NE）、100nmol·L-1血管紧张素II（ANG II）和30μmol·L-1三磷酸腺苷（ATP）存在时，RHR中SMCs的[Ca2+]i分别增加128%、132%、233%和152%。pra-C组与正常组相似。在含1mmol·L-1CaCl2的Hanks溶液中，SHR对照组SMCs的静息[Ca2+]i为（71±6）nmol·L-1。正常组SMCs的静息[Ca2+]i为（160±8）nmol·L-1，SHR为（362±18）nmol·L-1。在60mmol·L-1KCl和10μmol·L-1NE存在时，SHR中SMCs的[Ca2+]i分别增加235%和200%。pra-C组与正常组相似。SHR中SMCs的NO减少76%，pra-C组接近正常。pra-C改善了RHR胸主动脉的血管反应。
结论
这些结果表明，pra-C通过减小SMCs细胞大小、降低胶原含量、减少SMCs[Ca2+]i并增加NO生成来改善血管肥厚。
Zotero 插件