一种新的EID-1家族成员EID-2与组蛋白脱乙酰酶结合并抑制肌肉分化。
A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation.
作者信息
Miyake Satoshi, Yanagisawa Yuka, Yuasa Yasuhito
机构信息
Department of Molecular Oncology, Tokyo Medical and Dental University, Graduate School of Medicine and Dentistry, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
出版信息
J Biol Chem. 2003 May 9;278(19):17060-5. doi: 10.1074/jbc.M212212200. Epub 2003 Feb 13.
An EID-1 (E1A-like inhibitor of differentiation-1) inhibits differentiation by blocking the histone acetyltransferase activity of p300. Here we report a novel inhibitor of differentiation exhibiting homology to EID-1, termed EID-2 (EID-1-like inhibitor of differentiation-2). EID-2 inhibited MyoD-dependent transcription and muscle differentiation. Unlike EID-1, EID-2 did not block p300 activity. Interestingly, EID-2 associated with class I histone deacetylases (HDACs). The N-terminal portion of EID-2 was required for the binding to HDACs. This region was also involved in the transcriptional repression and nuclear localization, suggesting the importance of the involvement of HDACs in the EID-2 function. These results indicate a new family of differentiation inhibitors, although there are several differences in the biochemical mechanisms between EID-2 and EID-1.
E1A样分化抑制因子1（EID-1）通过阻断p300的组蛋白乙酰转移酶活性来抑制分化。在此，我们报告了一种与EID-1具有同源性的新型分化抑制因子，称为EID-2（EID-1样分化抑制因子2）。EID-2抑制MyoD依赖的转录和肌肉分化。与EID-1不同，EID-2不阻断p300活性。有趣的是，EID-2与I类组蛋白去乙酰化酶（HDACs）相关联。EID-2的N端部分是与HDACs结合所必需的。该区域也参与转录抑制和核定位，表明HDACs参与EID-2功能的重要性。这些结果表明存在一个新的分化抑制因子家族，尽管EID-2和EID-1之间在生化机制上存在一些差异。
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