红豆杉代谢组学：茉莉酸甲酯优先诱导杂种红豆杉细胞培养物中C-13位氧化紫杉烷类化合物的产生。

Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures.

作者信息

Ketchum Raymond E B, Rithner Christopher D, Qiu Deyou, Kim You Sun, Williams Robert M, Croteau Rodney B

机构信息

Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.

出版信息

Phytochemistry. 2003 Mar;62(6):901-9. doi: 10.1016/s0031-9422(02)00711-2.

DOI:10.1016/s0031-9422(02)00711-2

PMID:12590117

Abstract

Cells from suspension cultures of Taxus cuspidata were extracted with pentane as a source of relatively non-polar taxoids. Of the 13 taxoids identified in this fraction, eight were oxygenated at C-14 and two had not been previously described. These taxoids, along with existing taxoid standards, were employed to profile the metabolites of Taxus x media cv. Hicksii cell suspension cultures induced with methyl jasmonate to produce paclitaxel (Taxol). The majority of the taxoid metabolites produced in these induced cultures were oxygenated at C-13, and not C-14.

摘要

以戊烷作为相对非极性紫杉烷类化合物的来源，对东北红豆杉悬浮培养细胞进行提取。在该馏分中鉴定出的13种紫杉烷类化合物中，有8种在C-14位被氧化，2种是之前未被描述过的。这些紫杉烷类化合物与现有的紫杉烷类标准品一起，用于分析南方红豆杉cv. Hicksii细胞悬浮培养物经茉莉酸甲酯诱导产生紫杉醇（泰素）后的代谢产物。在这些诱导培养物中产生的大多数紫杉烷类代谢产物在C-13位被氧化，而非C-14位。