一项关于健康HIV血清阴性个体CD4和CD8计数观察的试点研究。

A pilot study on observations on CD4 & CD8 counts in healthy HIV seronegative individuals.

作者信息

Nag V L, Agarwal P, Venkatesh V, Rastogi P, Tandon R, Agrawal S K

机构信息

Department of Microbiology, King George's Medical College, Lucknow, India.

出版信息

Indian J Med Res. 2002 Aug;116:45-9.

PMID:12592989

Abstract

BACKGROUND & OBJECTIVES: CD4 T lymphocyte count is used to measure the progression of HIV infection and is recommended as part of the standard care of HIV infected person. Information on reference CD4 counts and CD4:CD8 ratio in healthy individuals is lacking in India. Therefore the present study was undertaken to obtain base-line data on CD4 counts and CD4:CD8 ratio of healthy population from north India and to assess the feasibility of using the values as reference in an extended larger study.

METHODS

In this pilot study 84 HIV negative healthy volunteers (56 males, 28 females) in the age group of 20-59 yr and who were willing to participate in the study were enrolled after proper counseling. Blood specimens were collected from each subject and processed for anti-HIV antibodies for exclusion of HIV. CD4 and CD8 counts of the samples were performed by fluorescence activated cell sorting (FACS).

RESULTS

The mean +/- SD of the absolute numbers of CD4 and CD8 lymphocytes/microliter was 763.6 +/- 226 and 547.5 +/- 190 in males and 797.9 +/- 263 and 567.7 +/- 250 in females. The range of the CD4 and CD8 counts was 365-1328 and 264-991 in males and 415-1257 and 224-1126 in females respectively. The mean +/- SD of the CD4:CD8 ratio was 1.47 +/- 0.42 in males and 1.52 +/- 0.45 in females.

INTERPRETATION & CONCLUSION: The results of this study showed that there is wide variability in CD4 count in our population, as is seen in studies reported from other parts of India. A large multicentric study could define the normal range for CD4 and CD8 counts and CD4:CD8 ratio in the Indian population.

摘要

背景与目的

CD4 T淋巴细胞计数用于衡量HIV感染的进展情况，并且被推荐作为HIV感染者标准治疗的一部分。印度缺乏健康个体的CD4参考计数及CD4:CD8比值的相关信息。因此，开展本研究以获取印度北部健康人群CD4计数及CD4:CD8比值的基线数据，并评估在一项更大规模的扩展研究中使用这些数值作为参考的可行性。

方法

在这项初步研究中，84名年龄在20至59岁之间、HIV检测呈阴性且愿意参与研究的健康志愿者，在经过适当咨询后被纳入研究。从每个受试者采集血样，并进行抗HIV抗体检测以排除HIV感染。样本的CD4和CD8计数通过荧光激活细胞分选（FACS）进行。

结果

男性CD4和CD8淋巴细胞每微升绝对数的平均值±标准差分别为763.6±226和547.5±190，女性分别为797.9±263和567.7±250。男性CD4和CD8计数范围分别为365 - 1328和264 - 991，女性分别为415 - 1257和224 - 1126。男性CD4:CD8比值的平均值±标准差为1.47±0.42，女性为1.52±0.45。