化脓性链球菌热原性外毒素C的锌依赖性主要组织相容性复合体II类结合位点对于最大程度的超抗原功能和毒性活性至关重要。
The zinc-dependent major histocompatibility complex class II binding site of streptococcal pyrogenic exotoxin C is critical for maximal superantigen function and toxic activity.
作者信息
Tripp Timothy J, McCormick John K, Webb Jennifer M, Schlievert Patrick M
机构信息
Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
出版信息
Infect Immun. 2003 Mar;71(3):1548-50. doi: 10.1128/IAI.71.3.1548-1550.2003.
Abstract
The cocrystal structure of streptococcal pyrogenic exotoxin C (SPE C) with HLA-DR2a (DRA0101,DRB50101) revealed a zinc-dependent interaction site through residues 167, 201, and 203 on SPE C and residue 81 on the beta-chain of HLA-DR2a (DRA0101,DRB50101). Mutation of these SPE C residues resulted in dramatically reduced biological activities. Thus, the zinc-dependent major histocompatibility complex II binding site is critical for maximal biological function of SPE C.
摘要
链球菌致热外毒素C（SPE C）与HLA - DR2a（DRA0101，DRB50101）的共晶体结构揭示了一个锌依赖性相互作用位点，该位点通过SPE C上的167、201和203位残基以及HLA - DR2a（DRA0101，DRB50101）β链上的81位残基形成。这些SPE C残基的突变导致生物活性显著降低。因此，锌依赖性主要组织相容性复合体II结合位点对SPE C的最大生物学功能至关重要。
相似文献
1
The zinc-dependent major histocompatibility complex class II binding site of streptococcal pyrogenic exotoxin C is critical for maximal superantigen function and toxic activity.
Infect Immun. 2003 Mar;71(3):1548-50. doi: 10.1128/IAI.71.3.1548-1550.2003.
2
Crystal structure of a superantigen bound to the high-affinity, zinc-dependent site on MHC class II.
Immunity. 2001 Jan;14(1):93-104. doi: 10.1016/s1074-7613(01)00092-9.
3
The superantigen streptococcal pyrogenic exotoxin C (SPE-C) exhibits a novel mode of action.
J Exp Med. 1997 Aug 4;186(3):375-83. doi: 10.1084/jem.186.3.375.
4
Immunological and biochemical characterization of streptococcal pyrogenic exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes.
J Immunol. 2001 Jun 1;166(11):6711-9. doi: 10.4049/jimmunol.166.11.6711.
5
Functional characterization of streptococcal pyrogenic exotoxin J, a novel superantigen.
Infect Immun. 2001 Mar;69(3):1381-8. doi: 10.1128/IAI.69.3.1381-1388.2001.
6
Crystal structure of the streptococcal superantigen SPE-C: dimerization and zinc binding suggest a novel mode of interaction with MHC class II molecules.
Nat Struct Biol. 1997 Aug;4(8):635-43. doi: 10.1038/nsb0897-635.
7
Structural basis for the recognition of superantigen streptococcal pyrogenic exotoxin A (SpeA1) by MHC class II molecules and T-cell receptors.
EMBO J. 1999 Jan 4;18(1):9-21. doi: 10.1093/emboj/18.1.9.
8
Lethal shock induced by streptococcal pyrogenic exotoxin A in mice transgenic for human leukocyte antigen-DQ8 and human CD4 receptors: implications for development of vaccines and therapeutics.
J Infect Dis. 2002 Aug 15;186(4):501-10. doi: 10.1086/341828. Epub 2002 Jul 19.
9
Zinc binding and dimerization of Streptococcus pyogenes pyrogenic exotoxin C are not essential for T-cell stimulation.
J Biol Chem. 2003 Mar 14;278(11):9885-95. doi: 10.1074/jbc.M206957200. Epub 2002 Dec 8.
10
Streptococcal pyrogenic exotoxin A (SPE A) superantigen induced production of hematopoietic cytokines, IL-12 and IL-13 by human peripheral blood mononuclear cells.
Microb Pathog. 1997 Nov;23(5):265-72. doi: 10.1006/mpat.1997.0155.
引用本文的文献
1
Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen.
PLoS One. 2014 Apr 15;9(4):e95200. doi: 10.1371/journal.pone.0095200. eCollection 2014.
2
The contribution of group A streptococcal virulence determinants to the pathogenesis of sepsis.
Virulence. 2014 Jan 1;5(1):127-36. doi: 10.4161/viru.26400. Epub 2013 Oct 9.
3
Staphylococcal superantigens in colonization and disease.
Front Cell Infect Microbiol. 2012 Apr 17;2:52. doi: 10.3389/fcimb.2012.00052. eCollection 2012.
4
Gram-positive bacterial superantigen outside-in signaling causes toxic shock syndrome.
FEBS J. 2011 Dec;278(23):4649-67. doi: 10.1111/j.1742-4658.2011.08151.x. Epub 2011 May 19.
5
The T cell receptor beta-chain second complementarity determining region loop (CDR2beta governs T cell activation and Vbeta specificity by bacterial superantigens.
J Biol Chem. 2011 Feb 11;286(6):4871-81. doi: 10.1074/jbc.M110.189068. Epub 2010 Dec 2.
6
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1).
Protein Sci. 2004 Sep;13(9):2285-90. doi: 10.1110/ps.04826804. Epub 2004 Aug 4.
本文引用的文献
1
Structures of two streptococcal superantigens bound to TCR beta chains reveal diversity in the architecture of T cell signaling complexes.
Structure. 2002 May;10(5):687-99. doi: 10.1016/s0969-2126(02)00759-1.
2
Toxic shock syndrome and bacterial superantigens: an update.
Annu Rev Microbiol. 2001;55:77-104. doi: 10.1146/annurev.micro.55.1.77.
3
Crystal structure of a superantigen bound to the high-affinity, zinc-dependent site on MHC class II.
Immunity. 2001 Jan;14(1):93-104. doi: 10.1016/s1074-7613(01)00092-9.
4
Development of streptococcal pyrogenic exotoxin C vaccine toxoids that are protective in the rabbit model of toxic shock syndrome.
J Immunol. 2000 Aug 15;165(4):2306-12. doi: 10.4049/jimmunol.165.4.2306.
5
Crystal structure of microbial superantigen staphylococcal enterotoxin B at 1.5 A resolution: implications for superantigen recognition by MHC class II molecules and T-cell receptors.
J Mol Biol. 1998 Mar 20;277(1):61-79. doi: 10.1006/jmbi.1997.1577.
6
Crystal structure of the streptococcal superantigen SPE-C: dimerization and zinc binding suggest a novel mode of interaction with MHC class II molecules.
Nat Struct Biol. 1997 Aug;4(8):635-43. doi: 10.1038/nsb0897-635.
7
Toxic shock syndrome toxin-1 complexed with a class II major histocompatibility molecule HLA-DR1.
Science. 1994 Dec 16;266(5192):1870-4. doi: 10.1126/science.7997880.
8
Staphylococcal enterotoxin A has two cooperative binding sites on major histocompatibility complex class II.
J Exp Med. 1995 Sep 1;182(3):711-20. doi: 10.1084/jem.182.3.711.
9
Bacterial pyrogenic exotoxins as superantigens.
Clin Microbiol Rev. 1995 Jul;8(3):411-26. doi: 10.1128/CMR.8.3.411.
10
Enhancement of host susceptibility to lethal endotoxin shock by staphylococcal pyrogenic exotoxin type C.
Infect Immun. 1982 Apr;36(1):123-8. doi: 10.1128/iai.36.1.123-128.1982.
Zotero 插件