一种功能遗传学方法表明，在体内，人类免疫缺陷病毒1型（HIV-1）的反式激活蛋白（Tat）与HIV-1反式激活应答元件RNA（TAR RNA）之间存在一种新的相互作用。

A functional genetic approach suggests a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Tat protein and HIV-1 TAR RNA in vivo.

作者信息

Lund Lars H, Wahren Britta, Garcia-Blanco Mariano A

机构信息

Medicine, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.

Departments of Molecular Genetics and Microbiology, Duke University Medical Center, Box 3053 (424 CARL), Research Drive, Durham, NC 27710, USA.

出版信息

J Gen Virol. 2003 Mar;84(Pt 3):603-606. doi: 10.1099/vir.0.18645-0.

DOI:10.1099/vir.0.18645-0

PMID:12604811

Abstract

Human immunodeficiency virus type 1 (HIV-1) Tat and human Cyclin T1 form a complex and together recognize the viral TAR RNA element with specificity. Using HIV-1/equine infectious anaemia virus TAR chimeras, we show that in addition to the well-characterized interaction with the bulge, Tat recognizes the distal stem and the loop of TAR. These data support previously proposed, but unproven, molecular models.

摘要

人类免疫缺陷病毒1型（HIV-1）的反式激活因子（Tat）与人类细胞周期蛋白T1（Cyclin T1）形成复合物，并共同特异性识别病毒TAR RNA元件。利用HIV-1/马传染性贫血病毒TAR嵌合体，我们发现，除了与凸起结构的相互作用已得到充分表征外，Tat还识别TAR的远端茎环结构。这些数据支持了先前提出但未经证实的分子模型。