草酰乙酸酮-烯醇互变异构酶的定位

Localization of oxalacetate keto-enol-tautomerase.

作者信息

Wesenberg J C, Chaudhari A, Annett R G

出版信息

Can J Biochem. 1976 Mar;54(3):233-7. doi: 10.1139/o76-036.

Abstract

The intracellular location of oxalacetate keto-enol-tautomerase (oxaloacetate keto-enolisomerase) (EC 5.3.2.2) has been determined in two types of animal cells, rat liver and pig kidney. Two fractionation procedures were adopted and modified to suit each type of tissue. One fractionation procedure gave the soluble phase, microsomal and mitochondrial fractions, while the other isolated the nuclear fraction. The tautomerase is distributed among the soluble phase, microsomes and mitochondria in both tissues. Fractionation efficiency was checked by determining percentage recoveries of enzymic activity and total protein after each step, by microscopy studies and by determining the distribution of several marker enzymes.

摘要

已在两种动物细胞（大鼠肝脏和猪肾脏）中确定了草酰乙酸酮-烯醇互变异构酶（草酰乙酸酮-烯醇异构酶）（EC 5.3.2.2）的细胞内定位。采用并修改了两种分级分离程序以适用于每种组织类型。一种分级分离程序得到了可溶性部分、微粒体和线粒体部分，而另一种则分离出了细胞核部分。互变异构酶分布于两种组织的可溶性部分、微粒体和线粒体中。通过在每个步骤后测定酶活性和总蛋白的回收率百分比、通过显微镜研究以及通过测定几种标记酶的分布来检查分级分离效率。