通过小角散射测定溶液中螺旋结构参数的分析模型：小角中子散射对RecA结构的比较

Analytical model for determination of parameters of helical structures in solution by small angle scattering: comparison of RecA structures by SANS.

作者信息

Lebedev D V, Baitin D M, Islamov A Kh, Kuklin A I, Shalguev V Kh, Lanzov V A, Isaev-Ivanov V V

机构信息

Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russia.

出版信息

FEBS Lett. 2003 Feb 27;537(1-3):182-6. doi: 10.1016/s0014-5793(03)00107-8.

DOI:10.1016/s0014-5793(03)00107-8

PMID:12606054

Abstract

The filament structures of the self-polymers of RecA proteins from Escherichia coli and Pseudomonas aeruginosa, their complexes with ATPgammaS, phage M13 single-stranded DNA (ssDNA) and the tertiary complexes RecA::ATPgammaS::ssDNA were compared by small angle neutron scattering. A model was developed that allowed for an analytical solution for small angle scattering on a long helical filament, making it possible to obtain the helical pitch and the mean diameter of the protein filament from the scattering curves. The results suggest that the structure of the filaments formed by these two RecA proteins, and particularly their complexes with ATPgammaS, is conservative.

摘要

通过小角中子散射比较了来自大肠杆菌和铜绿假单胞菌的RecA蛋白自聚物的丝状结构、它们与ATPγS、噬菌体M13单链DNA（ssDNA）的复合物以及三级复合物RecA::ATPγS::ssDNA。开发了一个模型，该模型允许对长螺旋丝上的小角散射进行解析求解，从而能够从散射曲线获得蛋白质丝的螺旋间距和平均直径。结果表明，这两种RecA蛋白形成的丝的结构，特别是它们与ATPγS的复合物的结构是保守的。