[P菌毛对人泌尿道脓尿及细菌定植的影响]

[Effect of P fimbriae on pyuria and bacterial colonization of the human urinary tract].

作者信息

Wullt B, Bergsten G, Samuelsson M, Svanborg C

机构信息

Department of Urology, Lund University Hospital, Lund, Sweden.

出版信息

Urologe A. 2003 Feb;42(2):233-7. doi: 10.1007/s00120-002-0287-y. Epub 2003 Jan 11.

DOI:10.1007/s00120-002-0287-y

PMID:12607092

Abstract

This study investigated the role of P fimbriae in colonization of Escherichia coli, host response, and bacterial persistence in humans. Human volunteers were inoculated intravesically with the nonadherent ABU isolate E. coli 83972 and with P fimbriated transformants of the same strain. During the following 24 h all urine samples, and thereafter daily samples, were collected for urine culture, analysis of neutrophil numbers, and cytokine concentrations (IL-6 and IL-8). The P fimbriated transformants showed enhanced bacterial colonization in comparison to E. coli 83972 and lowered the bacterial numbers needed for persistent bacteriuria. The P fimbriated transformants also lowered the bacterial numbers needed for a significant neutrophil and cytokine host response. We conclude that P fimbriae enhance bacterial colonization and trigger the host response in the human urinary tract.

摘要

本研究调查了P菌毛在大肠杆菌定植、宿主反应及细菌在人体内持续存在中的作用。将非黏附性无症状菌尿分离株大肠杆菌83972及其P菌毛转化株经膀胱内接种入人类志愿者体内。在接下来的24小时内，收集所有尿液样本，此后每天收集样本进行尿培养、中性粒细胞数量分析及细胞因子浓度（IL-6和IL-8）检测。与大肠杆菌83972相比，P菌毛转化株显示出更强的细菌定植能力，并降低了持续性菌尿所需的细菌数量。P菌毛转化株还降低了引发显著中性粒细胞和细胞因子宿主反应所需的细菌数量。我们得出结论，P菌毛可增强细菌在人类尿道中的定植并触发宿主反应。