Starling M R, Rosse C
Cell Tissue Kinet. 1976 Mar;9(2):191-204. doi: 10.1111/j.1365-2184.1976.tb01266.x.
Single and multiple injections of 3H-TdR have been used for measuring the rate of proliferation in morphologically defined cell populations of guinea-pig bone marrow that are committed to erythroid differentiation. The conclusions are based on the analysis of absolute cell numbers in the maturational compartments, the labeling and mitotic indices, labeled mitotic curves, pulse and chase grain counts over dividing and interphase cells, and on the rate or labeling during multiple, repeated injections of 3H-TdR. The average duration of S and the rate of cycling is similar in all maturational compartments of the erythrom. The majority of cells progress to the next maturational compartment by the time they divide for the second time. All proerythroblasts and basophilic erythroblasts are in cycle. Polychromatic erythroblasts incapable of incorporating 3H-TdR reach the orthochromatic population in the span of 5-6 hr. The orthochromatic population is renewed every 20-24 hr. The number of divisions between the proerythroblast and orthochromatic erythroblast does not exceed four and some cells may undergo only two divisions during the maturation pathway. Cell input from a progenitor cell population contributes to the maintenance of the erythron. The kinetic behavior of progenitor cells is similar to that of proerythroblasts. By the time of their second division, progenitor cells may reach either the proerythroblast or basophilic erythroblast compartments. The kinetic behavior of basophilic transitional cells corresponds to the predicted behavior of the erythroblast progenitor cell pool. Several of the conclusions are based on the assumption that grain count halving is the result of cell division. In view of the evidence discussed, this assumption in the present studies seems justified.
已使用单次和多次注射³H-TdR来测量豚鼠骨髓中致力于红细胞分化的形态学定义细胞群体的增殖速率。这些结论基于对成熟区室中绝对细胞数、标记指数和有丝分裂指数、标记有丝分裂曲线、分裂期和间期细胞上的脉冲和追踪颗粒计数的分析，以及基于多次重复注射³H-TdR期间的标记速率。红细胞生成的所有成熟区室中，S期的平均持续时间和循环速率相似。大多数细胞在第二次分裂时进入下一个成熟区室。所有原红细胞和嗜碱性红细胞都处于细胞周期中。无法掺入³H-TdR的多色红细胞在5-6小时内进入正染红细胞群体。正染红细胞群体每20-24小时更新一次。原红细胞和正染红细胞之间的分裂次数不超过四次，并且一些细胞在成熟途径中可能仅经历两次分裂。祖细胞群体的细胞输入有助于维持红细胞生成。祖细胞的动力学行为与原红细胞相似。在第二次分裂时，祖细胞可能进入原红细胞或嗜碱性红细胞区室。嗜碱性过渡细胞的动力学行为与红细胞祖细胞池的预测行为相符。其中一些结论基于颗粒计数减半是细胞分裂结果的假设。鉴于所讨论的证据，本研究中的这一假设似乎是合理的。
