Archibald A R, Heckels J E
Biochim Biophys Acta. 1975 Sep 16;406(1):60-7. doi: 10.1016/0005-2736(75)90042-5.
A nutritional mutant of Staphylococcus aureus H has been isolated and grown in media in which the only amino acids are arginine, cysteine, glutamic acid and proline. Walls of the bacteria grown in such media in continuous culture under potassium limitation differ in composition from walls of the bacteria grown in batch culture in rich nutrient broth in that they contain less glycine, the peptidoglycan component is less highly cross-linked and the teichoic acid component contains a reduced proportion of N-acetylglucosaminyl substituents. Walls of the potassium-limited bacteria retain the ability to bind bacteriophage 52a but are more susceptible to the action of lytic peptidases than are wall samples in which the peptidoglycan is more highly cross-linked. Teichoic acid was present in walls of the bacteria grown under phosphate limitation in the defined medium and these walls were also able to absorb bacteriophage 52a.
已分离出金黄色葡萄球菌H的一种营养突变体，并在仅含有精氨酸、半胱氨酸、谷氨酸和脯氨酸的培养基中培养。在钾限制条件下连续培养于此类培养基中的细菌细胞壁，其组成与在丰富营养肉汤中分批培养的细菌细胞壁不同，在于它们含有的甘氨酸较少，肽聚糖成分的交联程度较低，且磷壁酸成分中N - 乙酰葡糖胺取代基的比例降低。钾限制细菌的细胞壁保留了结合噬菌体52a的能力，但比肽聚糖交联程度更高的细胞壁样本更容易受到溶菌肽酶的作用。在限定培养基中磷限制条件下生长的细菌细胞壁中存在磷壁酸，且这些细胞壁也能够吸附噬菌体52a。
