L-α-羟酸氧化酶同工酶。纯化及分子特性
L-alpha-Hydroxyacid oxidase isozymes. Purification and molecular properties.
作者信息
Duley J A, Holmes R S
出版信息
Eur J Biochem. 1976 Mar 16;63(1):163-73. doi: 10.1111/j.1432-1033.1976.tb10219.x.
Abstract
L-alpha-Hydroxyacid oxidase isozymes from rat liver (A isozyme) and kidney (B isozyme) have been isolated in a high state of purity with specific activities of 61 and 14.7 microkatals per gram protein respectively. The subunit molecular weights determined by sodium dodecylsulphate polyacrylamide gel electrophoresis were 40000 +/- 3000; the mouse A and B isozymes were also partially purified and their subunit molecular weights shown to be 37000.
摘要
已从大鼠肝脏（A同工酶）和肾脏（B同工酶）中分离出高纯度的L-α-羟酸氧化酶同工酶，其比活性分别为每克蛋白质61和14.7微 katal。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定的亚基分子量为40000±3000；小鼠的A和B同工酶也被部分纯化，其亚基分子量显示为37000。
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