Michaelson D M, Duguid J R, Miller D L, Raftery M A
J Supramol Struct. 1976;4(3):419-25. doi: 10.1002/jss.400040312.
Dialysis of the purified acetylcholine receptor from Torpedo californica electroplax with lipids from the same organ results in a vesicular membrane system in which the receptor is embedded in the bilayer and oriented so that most of the neurotoxin-binding sites appear to be on the outer surface. The reconstituted vesicles are chemically excitable by acetylcholine and carbamylcholine, as measured by 22Na+ efflux. The excitability is specifically blocked by the antagonist alpha-bungarotoxin. These results demonstrate that the purified reconstituted receptor system not only can specifically bind neurotransmitter but can also trigger ion translocation. It therefore has the properties necessary to effect postsynaptic depolarization in vivo.
用来自加州电鳐电器官的脂质对纯化的乙酰胆碱受体进行透析，会形成一种囊泡膜系统，其中受体嵌入双层膜中，且其取向使得大多数神经毒素结合位点似乎位于外表面。通过22Na+外流测量发现，重构的囊泡对乙酰胆碱和氨甲酰胆碱具有化学兴奋性。这种兴奋性被拮抗剂α-银环蛇毒素特异性阻断。这些结果表明，纯化的重构受体系统不仅能特异性结合神经递质，还能触发离子转运。因此，它具有在体内实现突触后去极化所需的特性。
