[Effect of beta-interleukin on the content and proliferation of hemopoietic precursor cells in intact and irradiated long-term cultures of the bone marrow].

Effects of beta-leukine (human recombinant interleukin-1 beta) on the number and proliferative activity of early hemopoietic precursor cells (CFU-GM) and colonystimulating activity (GM-CSF) were studied in long-term cultures of the bone marrow. Studied were also recovery of CFU-GM, total number of myelocaryocytes and content of immature and mature granulocytes in response to beta-leukine after gamma-radiation of cultures in a dose 2.0 Gy. It was found that beta-leukine enhanced GM-CSF induction and elevated content of early CFU-GM in non-radiated long-term cultures of the bone marrow maximum 20 hours after introduction. 20 hours after introduction of beta-leukine proliferative activity of CFU-GM reached its maximum. The study of postradiation recovery in administration of beta-leukine in a dose 0.01 mcg/ml 10 min after radiation of long-term cultures of the bone marrow registered no significant stimulating effect of the drug on CFU-GN recovery, recovery of the number of myelocaryocytes and content of young immature and mature granulocytes.