sanC——一个参与产色链霉菌中多氧霉素生物合成的新基因。
sanC--a novel gene involved in nikkomycin biosynthesis in Streptomyces ansochromogenes.
作者信息
Xu J, Liu G, Tan H
机构信息
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
出版信息
Lett Appl Microbiol. 2003;36(4):234-8. doi: 10.1046/j.1472-765x.2003.01296.x.
AIMS
To investigate the genes involved in the nikkomycin biosynthesis and their molecular mechanism.
METHODS AND RESULTS
A 0.9 kbp SmaI fragment was cloned and sequenced which contains a complete open reading frame designated sanC (GenBank accession no. AF228522). In search of database, the deduced product of sanC was not homologous with any known proteins. The disruption and complementation of sanC showed that sanC is essential for nikkomycin biosynthesis in Streptomyces ansochromogenes.
CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: sanC is a novel and essential gene involved in nikkomycin biosynthesis in S. ansochromogenes.
目的
研究参与多氧霉素生物合成的基因及其分子机制。
方法与结果
克隆并测序了一个0.9 kbp的SmaI片段，其包含一个完整的开放阅读框，命名为sanC（GenBank登录号AF228522）。在数据库搜索中，sanC的推导产物与任何已知蛋白质均无同源性。sanC的破坏和互补表明，sanC对于产色链霉菌中多氧霉素的生物合成至关重要。
研究的结论、意义及影响：sanC是产色链霉菌中参与多氧霉素生物合成的一个新的必需基因。
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