Richman Douglas D, Wrin Terri, Little Susan J, Petropoulos Christos J
Department of Pathology, Veterans Affairs San Diego Healthcare System and the University of California at San Diego, La Jolla, CA 92093-0679, USA.
Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4144-9. doi: 10.1073/pnas.0630530100. Epub 2003 Mar 18.
A recombinant virus assay was used to characterize in detail neutralizing antibody responses directed at circulating autologous HIV in plasma. Examining serial plasma specimens in a matrix format, most patients with primary HIV infection rapidly generated significant neutralizing antibody responses to early (0-39 months) autologous viruses, whereas responses to laboratory and heterologous primary strains were often lower and delayed. Plasma virus continually and rapidly evolved to escape neutralization, indicating that neutralizing antibody exerts a level of selective pressure that has been underappreciated based on earlier, less comprehensive characterizations. These data argue that neutralizing antibody responses account for the extensive variation in the envelope gene that is observed in the early months after primary HIV infection.
采用重组病毒检测法详细表征针对血浆中循环自体HIV的中和抗体反应。以矩阵形式检测系列血浆样本，大多数原发性HIV感染患者迅速产生针对早期（0 - 39个月）自体病毒的显著中和抗体反应，而针对实验室毒株和异源原发性毒株的反应往往较低且延迟。血浆病毒持续快速进化以逃避中和，这表明中和抗体施加了一定程度的选择压力，而基于早期不太全面的表征，这种压力一直未得到充分认识。这些数据表明，中和抗体反应解释了原发性HIV感染后最初几个月包膜基因中观察到的广泛变异。
